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全长转录组测序和比较转录组分析评估 var. 在干旱和盐胁迫下的表现。

Full-Length Transcriptome Sequencing and Comparative Transcriptome Analysis to Evaluate Drought and Salt Stress in var. .

机构信息

Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China.

College of Forest Sciences, Nanjing Forestry University, Nanjing 210037, China.

出版信息

Genes (Basel). 2021 Mar 18;12(3):434. doi: 10.3390/genes12030434.

Abstract

var. ( var. ) is a perennial herb halophyte with salt and drought tolerance. In this study, full-length transcripts of var. were sequenced using the PacBio RSII sequencing platform. Moreover, the transcriptome was investigated under NaCl or polyethylene glycol (PEG) stress. Approximately 30.89 G subreads were generated and 31,195 unigenes were obtained by clustering the same isoforms by the PacBio RSII platform. A total of 15,466 differentially expressed genes (DEGs) were obtained under the two stresses using the Illumina platform. Among them, 9266 and 8390 DEGs were obtained under high concentrations of NaCl and PEG, respectively. In total, 3897 DEGs with the same expression pattern under the two stresses were obtained. The transcriptome expression profiles of var. under NaCl or PEG stress obtained in this study may provide a resource for the same and different response mechanisms against different types of abiotic stress. Furthermore, the stress-related genes found in this study can provide data for future molecular breeding.

摘要

变种(var. )是一种多年生盐生草本植物,具有耐盐和耐旱性。本研究使用 PacBio RSII 测序平台对 变种的全长转录本进行了测序。此外,还在 NaCl 或聚乙二醇(PEG)胁迫下对转录组进行了研究。通过 PacBio RSII 平台对相同的同工型进行聚类,共生成了约 30.89 G 的子读数,并获得了 31,195 个基因。使用 Illumina 平台在两种胁迫下共获得了 15466 个差异表达基因(DEGs)。其中,在高浓度 NaCl 和 PEG 下分别获得了 9266 和 8390 个 DEGs。总共获得了 3897 个在两种胁迫下具有相同表达模式的 DEGs。本研究中获得的 NaCl 或 PEG 胁迫下 变种的转录组表达谱可能为同种和不同种应对不同类型非生物胁迫的相同和不同反应机制提供资源。此外,本研究中发现的与胁迫相关的基因可为未来的分子育种提供数据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6cd/8002972/57fad0c34dd8/genes-12-00434-g001.jpg

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