Wu Hao, Su Wanlong, Shi Meijuan, Xue Xiaofang, Ren Haiyan, Wang Yongkang, Zhao Ailing, Li Dengke, Liu Mengjun
Shanxi Key Laboratory of Fruit Germplasm Creation and Utilization, Institute of Fruit Trees, Agricultural University of Shanxi, Taiyuan 030031, China.
College of Horticulture, Taigu Campus, Agricultural University of Shanxi, Jinzhong 030800, China.
Plants (Basel). 2023 Feb 14;12(4):858. doi: 10.3390/plants12040858.
Chinese jujube ( Mill.) originated in the Yellow River basin (YRB) of the Shanxi-Shaanxi region. The genomic C-value is a crucial indicator for plant breeding and germplasm evaluation. In this study, we used flow cytometry to determine the genomic C-values of jujube germplasms in the YRB of the Shanxi-Shaanxi region and evaluated their differences in different sub-regions. Of the 29 sub-regions, the highest and lowest variations were in Linxian and Xiaxian, respectively. The difference between jujube germplasms was highly significant (F = 14.89, < 0.0001) in Linxian. Cluster analysis showed that both cluster 2 and 4 belonged to Linxian, which were clearly separated from other taxa but were cross-distributed in them. Linxian County is an important gene exchange center in the YRB of the Shanxi-Shaanxi region. Principal component analysis showed that cluster 1 had low genomic C-values and single-fruit weights and cluster 2 had high genomic C-values and vitamin C contents. The genomic C-value was correlated with single-fruit weight and vitamin C content. In addition, the genomic C-value was used to predict fruit agronomic traits, providing a reference for shortening the breeding cycle and genetic diversity-related studies of jujube germplasm.
枣（Mill.）起源于山西-陕西地区的黄河流域（YRB）。基因组C值是植物育种和种质评价的关键指标。在本研究中，我们使用流式细胞术测定了山西-陕西地区黄河流域枣种质的基因组C值，并评估了它们在不同子区域的差异。在29个县中，变异最高和最低的分别是临县和夏县。临县枣种质间的差异极显著（F = 14.89，< 0.0001）。聚类分析表明，第2类和第4类均属于临县，它们与其他类群明显分开，但在其中交叉分布。临县是山西-陕西地区黄河流域重要的基因交流中心。主成分分析表明，第1类的基因组C值和单果重较低，第2类的基因组C值和维生素C含量较高。基因组C值与单果重和维生素C含量相关。此外，基因组C值被用于预测果实农艺性状，为缩短枣种质的育种周期和遗传多样性相关研究提供参考。
