Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Key Laboratory of Horticultural Crop (Fruit Trees) Biology and Germplasm Creation of the Ministry of Agriculture, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.
Viruses. 2020 Dec 25;13(1):25. doi: 10.3390/v13010025.
Chinese jujube ( Mill.) is a native fruit crop in China. Leaf mottle and dapple fruit disease is prevalent in cultivated jujube plants grown at Aksu in Xinjiang Uygur Autonomous Region of China. Jujube yellow mottle-associated virus (JYMaV), a tentative member in the genus , was recently identified from mottle-diseased jujube plants grown in Liaoning Province in China, but its incidence and genetic diversity in China is unknown. In this study, the genome sequences of three JYMaV isolates from two jujube cultivars and one jujube variant were determined by high-throughput sequencing (HTS) for small RNA and rRNA-depleted RNA coupled with RT-PCR assays. Comparison of these sequences together with sequences of the viral RNA segments derived by primer set 3C/5H-based RT-PCR revealed that genetic diversity was present in the virus populations and high sequence variation occurred at the non-translational regions of each of the viral genomic segments. Field investigation confirmed the close association of the virus with leaf mottle symptoms of jujube plants. Furthermore, this study revealed that P5 encoded in the viral RNA5 displayed a nuclear localization feature differing from the plasmodesma (PD) subcellular localization of the virus movement protein (P4), and the two proteins could interact with each other in the BiFC assays. Our study provides a snapshot of JYMaV genetic diversity in its natural hosts.
中国枣树（Mill.）是中国本土的水果作物。在中国新疆阿克苏地区，栽培枣树普遍发生枣树叶斑驳和果斑驳病。最近在中国辽宁省从患有斑驳病的枣树中鉴定出枣黄斑斑驳相关病毒（JYMaV），它是暂定属于病毒属的成员，但在中国其发病率和遗传多样性尚不清楚。在这项研究中，通过高通量测序（HTS）对来自两个枣树品种和一个枣树变体的三个 JYMaV 分离物的基因组序列进行了测定，该测序方法结合了小 RNA 和 rRNA 耗尽 RNA 与 RT-PCR 检测。将这些序列与通过引物 3C/5H 基于 RT-PCR 获得的病毒 RNA 片段序列进行比较表明，病毒种群中存在遗传多样性，每个病毒基因组片段的非翻译区都存在高度的序列变异。田间调查证实了该病毒与枣树叶片斑驳症状的密切关联。此外，本研究表明，病毒 RNA5 编码的 P5 具有不同于病毒运动蛋白（P4）的质膜定位特征，即核定位特征，并且这两种蛋白可以在 BiFC 测定中相互作用。我们的研究提供了 JYMaV 在其自然宿主中的遗传多样性的快照。
