Li Xueling, Cheng Yudou, Wang Ying, Yang Xiaohe, Wei Chuangqi, Guan Junfeng
Institute of Biotechnology and Food Sciences, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China.
Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051, China.
Plants (Basel). 2023 Feb 16;12(4):893. doi: 10.3390/plants12040893.
Ethylene is positively correlated with the anthocyanin accumulation in postharvest plum fruit, but the regulation mechanism has not been fully clarified. In this work, the 'Friar' plum fruit under different storage temperatures (0, 10 and 25 °C) and treatments (100.0 μL L ethylene and 1.0 μL L 1-MCP) were applied to study the relationship between anthocyanin accumulation and ethylene signal pathway. The fruits stored at 10 °C had higher ethylene production rate and more anthocyanin in flesh than those stored at 0 °C and 25 °C. Ten ethylene biosynthesis associated genes and forty-one ethylene signal transduction related genes were obtained from the previous transcriptome data. Among them, the expression levels of ethylene biosynthesis associated genes (, and ), and ethylene signal transduction related genes (, , , and ) were markedly higher in the fruits stored at 10 °C than those at 0 °C and 25 °C. Exogenous ethylene treatment enhanced while 1-MCP treatment inhibited the anthocyanin accumulation in the flesh under storage at 10 °C. In addition, exogenous ethylene treatment markedly increased the expression levels of , , , , , and in the flesh once it turning red, as well as the anthocyanin biosynthesis related genes (, , , , , and ), whereas 1-MCP treatment manifested the contrary effects. Correlation analysis indicated that there was a significant positive correlation between genes expression related to ethylene signal pathway and anthocyanin biosynthesis, except for . In conclusion, ethylene signal pathway is involved in the flesh reddening by up-regulating the anthocyanin biosynthesis related genes.
乙烯与采后李果实花青素积累呈正相关，但其调控机制尚未完全阐明。本研究以“Friar”李果实为试材，设置不同贮藏温度（0、10和25℃）和处理（100.0 μL L乙烯和1.0 μL L 1-甲基环丙烯），研究花青素积累与乙烯信号通路之间的关系。贮藏在10℃的果实比贮藏在0℃和25℃的果实具有更高的乙烯释放速率和果肉中更多的花青素。从前期转录组数据中获得了10个乙烯生物合成相关基因和41个乙烯信号转导相关基因。其中，乙烯生物合成相关基因（、和）以及乙烯信号转导相关基因（、、、和）在贮藏于10℃的果实中的表达水平显著高于0℃和25℃贮藏的果实。外源乙烯处理促进了10℃贮藏下果肉中花青素的积累，而1-甲基环丙烯处理则抑制了花青素的积累。此外，外源乙烯处理显著提高了果肉变红时、、、、和的表达水平，以及花青素生物合成相关基因（、、、、、和）的表达水平，而1-甲基环丙烯处理则表现出相反的效果。相关性分析表明，除外，乙烯信号通路相关基因表达与花青素生物合成之间存在显著正相关。综上所述，乙烯信号通路通过上调花青素生物合成相关基因参与果肉变红过程。
