Botany Department, Faculty of Science, Benha University, Benha, 13518, Egypt.
New Energy Department, School of Energy and Power Engineering, Jiangsu University, Zhenjiang, 212013, China.
Bioprocess Biosyst Eng. 2019 Mar;42(3):425-433. doi: 10.1007/s00449-018-2046-5. Epub 2018 Nov 21.
The isolated microalga Chlorella sorokiniana BENHA721_ABO4 was grown in Bold's basal medium (BBM) as a control, municipal wastewater (WW), and wastewater enriched with BBM elements (WW+). Cultivation in WW+ showed the highest cell number which represented 25.3 and 47.3% over that grown in WW and BBM, respectively. However, rapid growth in WW+ was accompanied by significant reduction in lipid content. Due to lipid accumulation in WW, it showed the maximum significant lipid productivity of 16.2 mg L day. Microalgae cultivation in WW for 10 days showed 74.2, 83.3, and 78.0% removal efficiency for NO-N, NH-N and TP, respectively. In addition, growth in WW significantly reduced polyunsaturated fatty acids by 36.0% with respect to BBM in favor of monounsaturated fatty acids. The present results confirmed that C. sorokiniana isolate BENHA721_ABO4 grown in secondary effluent municipal wastewater offers real potential for future application in wastewater treatment and biodiesel production.
分离出的小球藻 Chlorella sorokiniana BENHA721_ABO4 在 Bold 基础培养基 (BBM) 中生长作为对照，城市污水 (WW) 和富含 BBM 元素的废水 (WW+)。在 WW+中培养表现出最高的细胞数量，分别比在 WW 和 BBM 中生长高出 25.3%和 47.3%。然而，WW+中的快速生长伴随着脂质含量的显著降低。由于 WW 中存在脂质积累，其表现出最高的显著脂质生产力为 16.2 mg L day。在 WW 中培养 10 天，NO-N、NH-N 和 TP 的去除效率分别达到 74.2%、83.3%和 78.0%。此外，与 BBM 相比，WW 中的生长显著降低了 36.0%的多不饱和脂肪酸，有利于单不饱和脂肪酸。本研究结果证实，从二级出水城市污水中分离出的小球藻 BENHA721_ABO4 对未来在废水处理和生物柴油生产中的应用具有很大的潜力。
