Department of Health Sciences, University of Piemonte Orientale "A. Avogadro", Novara.
Eur J Histochem. 2023 Feb 23;67(1):3679. doi: 10.4081/ejh.2023.3679.
Hydrogels based on various polymeric materials have been successfully developed in recent years for a variety of skin applications. Several studies have shown that hydrogels with regenerative, antibacterial, and antiinflammatory properties can provide faster and better healing outcomes, particularly in chronic diseases where the normal physiological healing process is significantly hampered. Various experimental tests are typically performed to assess these materials' ability to promote angiogenesis, re-epithelialization, and the production and maturation of new extracellular matrix. Immunohistochemistry is important in this context because it allows for the visualization of in situ target tissue factors involved in the various stages of wound healing using antibodies labelled with specific markers detectable with different microscopy techniques. This review provides an overview of the various immunohistochemical techniques that have been used in recent years to investigate the efficacy of various types of hydrogels in assisting skin healing processes. The large number of scientific articles published demonstrates immunohistochemistry's significant contribution to the development of engineered biomaterials suitable for treating skin injuries.
近年来，基于各种聚合材料的水凝胶已成功开发出来，用于各种皮肤应用。多项研究表明，具有再生、抗菌和抗炎特性的水凝胶可以提供更快、更好的愈合效果，特别是在正常生理愈合过程受到显著阻碍的慢性疾病中。通常需要进行各种实验测试来评估这些材料促进血管生成、再上皮化以及新细胞外基质的产生和成熟的能力。免疫组织化学在这方面很重要，因为它允许使用标记有特定标记物的抗体来可视化原位靶组织因子，这些标记物可以使用不同的显微镜技术检测到。本综述概述了近年来用于研究各种类型水凝胶在辅助皮肤愈合过程中的功效的各种免疫组织化学技术。大量发表的科学文章表明，免疫组织化学对开发适合治疗皮肤损伤的工程生物材料做出了重大贡献。
