Eye Hospital and School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China.
Oujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Front Endocrinol (Lausanne). 2023 Feb 9;14:1049326. doi: 10.3389/fendo.2023.1049326. eCollection 2023.
To clarify the effects of acute hyperglycemia on the responses of choroidal structural components and vascularity index during light modulation in healthy participants using techniques including image binarization and artificial intelligence (AI) segmentation based on swept-source optical coherence tomography (SS-OCT).
Twenty-four eyes of 24 healthy participants were imaged at different stages after ambient light, 40 min of dark adaptation, and 5 min of light adaptation in two imaging sessions: control and after receiving 75 g of oral glucose solution. The choroidal structural parameters, including luminal volume (LV), stromal volume (SV), total choroidal volume (TCV), and choroidal vascularity index (CVI) within a 6 mm area were determined using a custom algorithm based on image binarization and AI segmentation of SS-OCT. These measurements were compared among the conditions after adjusting for axial length, age to identify the differences.
In the dark, CVI decreased (-0.36 ± 0.09%) significantly in acute hyperglycemia compared to the control condition. During the transition to ambient light, there was an increasing trend in the choroidal parameters compared with the control experiment. However, only TCV (0.38 ± 0.17 mm) and LV (0.27 ± 0.10 mm) showed a significant increase at the time point of 5 min after ambient light.
Analysis of choroidal structural parameters and CVI based on SS-OCT images is a potentially powerful method to objectively reflect subtle changes in neurovascular coupling between the choroid and photoreceptor during dark adaptation.
利用基于扫频源光相干断层扫描（SS-OCT）的图像二值化和人工智能（AI）分割技术，明确急性高血糖对健康参与者在光调制期间脉络膜结构成分和血管指数反应的影响。
在两次成像过程中，对 24 名健康参与者的 24 只眼，分别在环境光下、暗适应 40 分钟后以及亮适应 5 分钟后进行成像，记录其脉络膜结构参数，包括在 6mm 区域内的管腔容积（LV）、基质容积（SV）、总脉络膜容积（TCV）和脉络膜血管指数（CVI），这些参数是利用 SS-OCT 的图像二值化和 AI 分割技术，通过一个定制算法进行测量。对这些参数进行分析，比较在接受 75g 口服葡萄糖溶液前后不同条件下的差异，同时还调整了眼轴和年龄的因素。
在暗环境中，与对照条件相比，急性高血糖时 CVI 显著降低（-0.36±0.09%）。在向环境光过渡期间，与对照实验相比，脉络膜参数呈上升趋势。然而，只有在环境光后 5 分钟时，TCV（0.38±0.17mm）和 LV（0.27±0.10mm）表现出明显的增加。
基于 SS-OCT 图像的脉络膜结构参数和 CVI 分析是一种潜在的强大方法，可以客观地反映暗适应期间脉络膜和光感受器之间神经血管耦联的细微变化。
