Department of Biological Sciences, Higher Teacher Training College, University of Yaounde 1, Yaounde, Cameroon.
Laboratoire de Biochimie et Chimie Appliquées, Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso.
Pan Afr Med J. 2022 Jul 21;42:222. doi: 10.11604/pamj.2022.42.222.33149. eCollection 2022.
the leaves of Vernonia amygdalina (V. amygdalina) are consumed as food in sub-Saharan Africa (SSA). In traditional medicine, this plant is widely used in the treatment of cancer and diabetes mellitus. In the present study, we evaluated the antihyperglycemic and the antiproliferative activities of the hydroalcoholic extract of V. amygdalina leaves (HAEVa).
we conducted an experimental descriptive and analytical study with a prospective data collection from May 2019 to July 2020. For the in vivo study, the experiments were carried out on albino male rats of Wistar strain (Rattus norvegicus). Antihyperglycemic activity was performed in vivo in dexamethasone-induced insulin-resistant rats using the oral glucose tolerance test (OGTT). The biocompatibility and the antiproliferative activity of extract were performed in vitro respectively on rabbit primary dermal fibroblasts (RPDF) and human osteosarcoma MG-63 cells using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The data were analyzed with the GraphPad Prism software version 5.0.3. The statistical analyses were obtained by the analysis of variance (ANOVA), followed by Bonferroni´s post-test. P<0.05 was considered as the minimal level of statistical significance.
regarding to the antiproliferative investigation, extract at 125, 250 μg/mL exhibited a significant cytotoxic effect on human osteosarcoma MG-63 compared to the vehicle (p<0.001) in a dose-response manner after 24h, 48h of exposure to HAEVa. Interestingly, HAEVa in concentrations of 125 and 250μg/ml showed no cytotoxicity (p>0.05) on RPDF after the different times of exposure. However, HAEVa in a high concentration of 500 μg/mL wasn´t biocompatible with RPDF. HAEVa also prevented postprandial blood glucose level in dexamethasone-induced insulin-resistant rats at both doses tested (p>0.05 and p<0.01 at doses of 50 and 100 mg/kg respectively).
the results of this study suggest that HAEVa has antiproliferative properties on MG-63 osteosarcoma in vitro and also inhibits in vivo the postprandial blood glucose level in dexamethasone-induced insulin-resistant rats.
在撒哈拉以南非洲地区（SSA），斑鸠菊属（V. amygdalina）的叶子被当作食物食用。在传统医学中，这种植物被广泛用于治疗癌症和糖尿病。在本研究中，我们评估了斑鸠菊属叶水醇提取物（HAEVa）的降血糖和抗增殖活性。
我们进行了一项实验性描述性和分析性研究，前瞻性地收集了 2019 年 5 月至 2020 年 7 月的数据。在体内研究中，我们使用口服葡萄糖耐量试验（OGTT）在接受地塞米松诱导的胰岛素抵抗大鼠中进行了体内降血糖活性研究。体外分别用兔原代真皮成纤维细胞（RPDF）和人骨肉瘤 MG-63 细胞进行了提取物的生物相容性和抗增殖活性研究，采用 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）法。数据使用 GraphPad Prism 软件版本 5.0.3 进行分析。统计分析采用方差分析（ANOVA），然后采用 Bonferroni 事后检验。P<0.05 被认为是统计学意义的最小水平。
在抗增殖研究中，与载体相比，提取物在 125、250μg/ml 浓度下，在 24、48 小时的暴露后，对人骨肉瘤 MG-63 具有显著的细胞毒性作用（p<0.001），呈剂量依赖性。有趣的是，HAEVa 在 125 和 250μg/ml 的浓度下，在不同的暴露时间后，对 RPDF 没有细胞毒性（p>0.05）。然而，HAEVa 在高浓度 500μg/ml 时与 RPDF 不兼容。HAEVa 还能防止两种剂量（50 和 100mg/kg 时分别为 p>0.05 和 p<0.01）的地塞米松诱导的胰岛素抵抗大鼠餐后血糖水平升高。
本研究结果表明，HAEVa 具有体外抗 MG-63 骨肉瘤增殖的特性，并且还能抑制地塞米松诱导的胰岛素抵抗大鼠的餐后血糖水平。
