Wu Hui, Wang Ziyi, Yu Yanchun, Zhang Yanli
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
Bio Protoc. 2023 Feb 20;13(4):e4611. doi: 10.21769/BioProtoc.4611.
Chloroplast movement has been observed and analyzed since the 19 century. Subsequently, the phenomenon is widely observed in various plant species such as fern, moss, , and . However, chloroplast movement in rice is less investigated, presumably due to the thick wax layer on its leaf surface, which reduces light sensitivity to the point that it was previously believed that there was no light-induced movement in rice. In this study, we present a convenient protocol suitable for observing chloroplast movement in rice only by optical microscopy without using special equipment. It will allow researchers to explore other signaling components involved in chloroplast movement in rice.
自19世纪以来，叶绿体运动就已被观察和分析。随后，这种现象在各种植物物种中广泛观察到，如蕨类植物、苔藓等。然而，水稻中的叶绿体运动较少被研究，可能是由于其叶片表面的蜡质层较厚，这降低了光敏感性，以至于以前人们认为水稻中不存在光诱导运动。在本研究中，我们提出了一种简便的方法，仅通过光学显微镜就能观察水稻中的叶绿体运动，而无需使用特殊设备。这将使研究人员能够探索水稻中叶绿体运动所涉及的其他信号成分。
