Medical Supplies Center of Chinese PLA General Hospital, Beijing 100853, China.
Eighth Medical Center of Chinese PLA General Hospital, Beijing 100091, China.
Anal Cell Pathol (Amst). 2023 Feb 15;2023:6306358. doi: 10.1155/2023/6306358. eCollection 2023.
Phagocytic ability of macrophage is responsible for tuberculosis infection. Nicotine has been shown to attenuate the phagocytic ability of macrophage; however, the underlying mechanism remains unclear. Here, we demonstrated that nicotine increased the message RNA (mRNA) and protein expression of signal regulatory protein alpha (SIRP) and enhanced the stability of SIRP mRNA in macrophage. Nicotine decreased the expression of microRNA (miR)-296-3p, which directly targeted the 3'-untranslated region (3'-UTR) of SIRP mRNA in macrophage. Furthermore, nicotine inhibited the phagocytic ability of macrophage by regulating the miR-296-3p-SIRP axis. Moreover, nicotine decreased miR-296-3p expression via increasing c-Myc expression in macrophage. Together, we found that nicotine attenuate the phagocytic ability of macrophage by regulating the c-Myc-miR-296-3p-SIRP signal.
巨噬细胞的吞噬能力是导致结核病感染的原因。已有研究表明，尼古丁可减弱巨噬细胞的吞噬能力；然而，其潜在机制尚不清楚。在这里，我们证明尼古丁可增加信号调节蛋白α（SIRP）的信使 RNA（mRNA）和蛋白表达，并增强巨噬细胞中 SIRP mRNA 的稳定性。尼古丁降低了 microRNA（miR）-296-3p 的表达，miR-296-3p 可直接靶向 SIRP mRNA 的 3'非翻译区（3'-UTR）。此外，尼古丁通过调节 miR-296-3p-SIRP 轴来抑制巨噬细胞的吞噬能力。此外，尼古丁通过增加巨噬细胞中 c-Myc 的表达来降低 miR-296-3p 的表达。综上所述，我们发现尼古丁通过调节 c-Myc-miR-296-3p-SIRP 信号来减弱巨噬细胞的吞噬能力。
