耐药性和毒力因子与血细胞计数和凝血指标的相关性分析。

Correlation Analysis of Drug Resistance and Virulence Factors with Blood Cell Counts and Coagulation Indexes.

机构信息

Department of Laboratory Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, Jiangsu, China.

Department of Clinical Laboratory, Yancheng Maternity and Child Health Hospital, 31 Century Avenue East Road, Yancheng 224000, Jiangsu, China.

出版信息

Int J Clin Pract. 2023 Feb 15;2023:8768152. doi: 10.1155/2023/8768152. eCollection 2023.

OBJECTIVE

The influence of different variants on blood cells and coagulation system was evaluated by investigating the carrying status of drug resistance genes and virulence genes of methicillin-resistant (MRSA) and methicillin-sensitive (MSSA).

METHODS

A total of 105 blood culture-derived strains were collected. The carrying status of drug resistance genes mecA and three virulence genes , and was analyzed by polymerase chain reaction (PCR). The changes in routine blood routine counts and coagulation indexes of patients infected with different strains were analyzed.

RESULTS

The results showed that the positive rate of mecA was consistent with that of MRSA. Virulence genes and were detected only in MRSA. Compared with MSSA, patients infected with MRSA or MSSA patients infected with virulence factor, leukocyte count and neutrophil count in peripheral blood were significantly increased, and the platelet count decreased to a higher degree. Part thromboplastin time increased, D-dimer increased, but fibrinogen content decreased more. The changes of erythrocyte and hemoglobin had no significant correlation with whether carried virulence genes.

CONCLUSION

The detection rate of MRSA in patients with positive in blood culture had exceeded 20%. The detected MRSA bacteria carried three virulence genes, , and , which were more likely than MSSA. MRSA, which carries two virulence genes, is more likely to cause clotting disorders.

目的

通过研究耐甲氧西林金黄色葡萄球菌（MRSA）和甲氧西林敏感金黄色葡萄球菌（MSSA）耐药基因和毒力基因的携带状态，评估不同变异体对血细胞和凝血系统的影响。

方法

共收集 105 株血培养分离株。采用聚合酶链反应（PCR）分析耐药基因 mecA 和三个毒力基因、和的携带情况。分析不同菌株感染患者常规血常规计数和凝血指标的变化。

结果

结果显示 mecA 的阳性率与 MRSA 一致。毒力基因仅在 MRSA 中检测到。与 MSSA 相比，MRSA 或携带毒力因子的 MSSA 患者感染后，外周血白细胞计数和中性粒细胞计数明显增加，血小板计数下降程度更高。部分凝血活酶时间增加，D-二聚体增加，但纤维蛋白原含量下降更明显。红细胞和血红蛋白的变化与是否携带毒力基因无明显相关性。