鼻腔固有免疫蛋白质组在盐水冲洗后的变化：健康个体的初步研究。
The Nasal Innate Immune Proteome After Saline Irrigation: A Pilot Study in Healthy Individuals.
机构信息
College of Medicine and Public Health, Flinders University, Bedford Park, SA, Australia.
Proteomics Facility, College of Medicine and Public Health, Flinders University, Bedford Park, SA, Australia.
出版信息
Am J Rhinol Allergy. 2023 Jul;37(4):419-428. doi: 10.1177/19458924231159176. Epub 2023 Feb 27.
BACKGROUND
Previous research has shown diminished nasal immune function following nasal saline irrigation (NSI), returning to baseline at 6 hours. The aim of this study was to examine the immune nasal proteome before and after 14 days of nasal irrigation.
METHODS
Seventeen healthy volunteers received either isotonic (IsoSal) or low salt (LowNa) NSI. Nasal secretions were collected before and 30 min after NSI at baseline and again after 14 days. Specimens were analyzed using mass spectrometry to detect proteins of relevance to nasal immune function.
RESULTS
One thousand eight hundred and sixty-five proteins were identified with significant changes in 71 proteins, of which 23 were identified as part of the innate immune system. Baseline analysis demonstrated an increase of 9 innate proteins after NSI, most after IsoSal. After 14 days, a greater increase in innate peptides was present, with most now in the LowNa group. When NSI solutions were compared, a significant increase in 4 innate proteins, including a 211% in lysozyme, was detected in the LowNa group.
CONCLUSION
LowNa NSI demonstrates evidence of improving the innate immune secretions, especially lysozyme, in healthy volunteers.
背景
先前的研究表明，鼻腔盐水冲洗（NSI）后鼻腔免疫功能减弱，6 小时后恢复到基线水平。本研究旨在检测 NSI 前后 14 天鼻腔免疫蛋白质组。
方法
17 名健康志愿者接受等渗（IsoSal）或低盐（LowNa）NSI。在基线时以及 NSI 后 30 分钟和 14 天后收集鼻分泌物。使用质谱分析法分析标本，以检测与鼻腔免疫功能相关的蛋白质。
结果
鉴定出 1865 种蛋白质，其中 71 种蛋白质发生了显著变化，其中 23 种被鉴定为固有免疫系统的一部分。基线分析表明，NSI 后 9 种固有蛋白增加，大多数是 IsoSal 后增加。14 天后，固有肽的增加更为明显，大多数出现在 LowNa 组。当比较 NSI 溶液时，在 LowNa 组中检测到 4 种固有蛋白（包括溶菌酶增加 211%）显著增加。
结论
低渗 NSI 可改善健康志愿者的固有免疫分泌物，尤其是溶菌酶。
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