Toll样受体4（TLR4）介导的髓样分化因子88（MyD88）/肿瘤坏死因子受体相关因子6（TRAF6）/核因子κB（NF-κB）信号通路及多聚免疫球蛋白受体（pIgR）在肠炎沙门氏菌感染雏鸡肠道中的表达作用
The role of TLR4-mediated MyD88/TRAF6/NF-κB signaling and pIgR intestinal expression in chicks during Salmonella enteritidis infection.
作者信息
Zhang C, Ding Y, Liu Y F, Wang H B, Wang X J, Wang S Y, Sun Z Y, Li D J
机构信息
Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, China.
出版信息
Vet Immunol Immunopathol. 2023 Apr;258:110563. doi: 10.1016/j.vetimm.2023.110563. Epub 2023 Feb 15.
To observe the effect of Salmonella enteritidis (SE)-induced inflammation on pIgR expression in jejunum and ileum. Salmonella enteritidis was orally administered to 7-day old Hyline chicks, which were killed after 1d,3d,7d and 14d. The mRNA expression of TLR4,MyD88,TRAF6,NF-κB, and pIgR was detected by real-time RT-PCR, and pIgR protein was detected by Western blotting. The TLR4 signaling pathway was activated, the mRNA expression of the pIgR in jejunum and ileum was increased, and pIgR protein in jejunum and ileum was up-regulated by SE. In SE-treated chicks,the pIgR in jejunum and ileum was up-regulated on mRNA,and protein level,associated with activation of the TRL4-mediated MyD88/TRAF6/NF-κB signaling pathway, which identifies this as a novel pIgR-related pathway to TLR4 activation.
观察肠炎沙门氏菌（SE）诱导的炎症对空肠和回肠中多聚免疫球蛋白受体（pIgR）表达的影响。将肠炎沙门氏菌口服接种于7日龄海兰雏鸡，分别于接种后1天、3天、7天和14天处死雏鸡。采用实时逆转录聚合酶链反应（RT-PCR）检测Toll样受体4（TLR4）、髓样分化因子88（MyD88）、肿瘤坏死因子受体相关因子6（TRAF6）、核因子κB（NF-κB）和pIgR的mRNA表达，采用蛋白质印迹法检测pIgR蛋白。SE激活了TLR4信号通路，空肠和回肠中pIgR的mRNA表达增加，空肠和回肠中的pIgR蛋白上调。在SE处理的雏鸡中，空肠和回肠中pIgR的mRNA和蛋白水平上调，这与TRL4介导的MyD88/TRAF6/NF-κB信号通路的激活有关，这表明这是一条与TLR4激活相关的新的pIgR相关通路。
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