Yang Heyun, Li Xiaoliang, Guo MengHan, Cao Xin, Zheng Xing, Bao Dongguan
State Key Laboratory of Eco-hydraulics in North west Arid Region, Xi'an University of Technology, Xi'an 710048, China; Department of Municipal and Environmental Engineering, School of Water Resources and Hydro-Electric Engineering, Xi'an University of Technology, Xi'an, Shaanxi 710048, China.
State Key Laboratory of Eco-hydraulics in North west Arid Region, Xi'an University of Technology, Xi'an 710048, China; Department of Municipal and Environmental Engineering, School of Water Resources and Hydro-Electric Engineering, Xi'an University of Technology, Xi'an, Shaanxi 710048, China.
Mar Pollut Bull. 2023 Apr;189:114745. doi: 10.1016/j.marpolbul.2023.114745. Epub 2023 Feb 26.
Herein, the toxicity of 4 MPs and additives released from MPs during UV-aging was quantitatively evaluated by the transcriptional effect level index (TELI) based on E. coli whole-cell microarray assay, and MPs-antibiotics complex pollutants. Results showed that MPs and these additives had high toxicity potential, the maximum TELI was 5.68/6.85 for polystyrene (PS)/bis(2-ethylhexyl) phthalate (DEHP). There were many similar toxic pathways between MPs and additives, indicating that part of the toxicity risk of MPs was caused by the release of additives. MPs were compounded with antibiotics, the toxicity value changed significantly. The TELI values of amoxicillin (AMX) + polyvinyl chloride (PVC) and ciprofloxacin (CIP) + PVC were as high as 12.30 and 14.58 (P < 0.05). Three antibiotics all decreased the toxicity of PS and had little effect on polypropylene (PP) and polyethylene (PE). The combined toxicity mechanism of MPs and antibiotics was very complicated, and the results could be divided into four types: MPs (PVC/PE + CIP), antibiotics (PVC + TC, PS + AMX/ tetracycline (TC)/CIP, PE + TC), both (PP + AMX/TC/CIP), or brand-new mechanisms (PVC + AMX).
在此，基于大肠杆菌全细胞微阵列分析，通过转录效应水平指数（TELI）对4种微塑料及其在紫外线老化过程中释放的添加剂的毒性以及微塑料 - 抗生素复合污染物进行了定量评估。结果表明，微塑料及其添加剂具有较高的潜在毒性，聚苯乙烯（PS）/邻苯二甲酸二（2-乙基己基）酯（DEHP）的最大TELI为5.68/6.85。微塑料与添加剂之间存在许多相似的毒性途径，这表明微塑料的部分毒性风险是由添加剂的释放引起的。微塑料与抗生素复合后，毒性值发生显著变化。阿莫西林（AMX）+聚氯乙烯（PVC）和环丙沙星（CIP）+PVC的TELI值分别高达12.30和14.58（P<0.05）。三种抗生素均降低了PS的毒性，而对聚丙烯（PP）和聚乙烯（PE）影响较小。微塑料与抗生素复合的毒性机制非常复杂，结果可分为四种类型：微塑料（PVC/PE + CIP）、抗生素（PVC + 四环素（TC）、PS + AMX/TC/CIP、PE + TC）、两者皆有（PP + AMX/TC/CIP）或全新机制（PVC + AMX）。
