Zhu Jihua, Miao Congcong, Wang Xicun
College of Chemistry and Chemical Engineering, Qinghai Normal University, Xining, Qinghai 810008, PR China; College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, Gansu 730070, P. R. China; Academy of Plateau Science and Sustainability, People's Government of Qinghai Province & Beijing Normal University, Xining, Qinghai 810008, P. R. China.
College of Chemistry and Chemical Engineering, Qinghai Normal University, Xining, Qinghai 810008, PR China; College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, Gansu 730070, P. R. China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Jun 5;294:122546. doi: 10.1016/j.saa.2023.122546. Epub 2023 Feb 24.
Hypochlorous acid (HClO) plays an essential role in biological systems. The characteristics of potent oxidization and short lifetime make it challenging to detect specifically from other reactive oxygen species (ROS) at cellular levels. Therefore, its detection and imaging with high selectivity and sensitivity are of great significance. Herein a turn-on HClO fluorescent probe (named RNB-OCl) with boronate ester as the recognition site was designed and synthesized. The RNB-OCl displayed good selective and ultrasensitive to HClO with a low detection limit of 1.36 nM by the intramolecular charge transfer (ICT)-fluorescence resonance energy transfer (FRET) dual mechanism in reducing the fluorescence background and improving the sensitivity. In addition, the role of the ICT-FRET was further demonstrated by time-dependent density functional theory (TD-DFT) calculations. Furthermore, the probe RNB-OCl was successfully employed for imaging HClO in living cells.
次氯酸（HClO）在生物系统中起着至关重要的作用。其强大的氧化特性和较短的寿命使得在细胞水平上从其他活性氧（ROS）中特异性检测它具有挑战性。因此，对其进行高选择性和高灵敏度的检测及成像具有重要意义。在此，设计并合成了一种以硼酸酯为识别位点的开启型HClO荧光探针（命名为RNB-OCl）。通过分子内电荷转移（ICT）-荧光共振能量转移（FRET）双重机制，RNB-OCl对HClO表现出良好的选择性和超灵敏性，检测限低至1.36 nM，可降低荧光背景并提高灵敏度。此外，通过含时密度泛函理论（TD-DFT）计算进一步证明了ICT-FRET的作用。此外，探针RNB-OCl成功用于活细胞中HClO的成像。
