大鼠胎盘中的细胞溶质脂肪酸结合蛋白

Cytosolic fatty acid binding proteins in rat placenta.

作者信息

Brandes R, Arad R, Barash V

机构信息

Institute of Biochemistry, Hebrew University, Hadassah Medical School, Jerusalem, Israel.

出版信息

Placenta. 1987 Jul-Aug;8(4):347-50. doi: 10.1016/0143-4004(87)90062-2.

DOI:10.1016/0143-4004(87)90062-2

PMID:3684966

Abstract

Placentae from 21 days pregnant rats were assayed for long-chain fatty acid binding activity. Oleate binding activity, associated with a low-molecular-weight protein fraction resembling liver fatty acid binding protein (FABP or 'Z' protein), could be demonstrated after removal of serum albumin by fractionation of the placental cytosol on Sephadex G-75. The fatty acid binding activity in the placenta was 2 to 4 per cent of the FABP activity present in rat liver.

摘要

对怀孕21天的大鼠胎盘进行长链脂肪酸结合活性检测。通过在葡聚糖凝胶G - 75上对胎盘细胞溶质进行分级分离去除血清白蛋白后，可证明与一种类似于肝脏脂肪酸结合蛋白（FABP或“Z”蛋白）的低分子量蛋白质组分相关的油酸结合活性。胎盘中的脂肪酸结合活性是大鼠肝脏中FABP活性的2%至4%。