Brandes R, Arad R
Biochim Biophys Acta. 1983 Feb 7;750(2):334-9. doi: 10.1016/0005-2760(83)90037-1.
The binding of [1-(14)C]oleate to rat liver cytosol was studied, using gel filtration on Sephadex G-75 and G-50. In liver cytosols from control rats, most of the high-affinity oleate binding was in the region of 12 000-dalton proteins. In liver cytosols from diabetic and starved rats, a second peak of radioactivity appeared in the void volume. This peak was shown to be associated with a component having the molecular weight of 400 000. Evidence suggesting that a change in the composition of cytosolic binding proteins is involved is presented.
利用Sephadex G - 75和G - 50进行凝胶过滤，研究了[1 - （14）C]油酸酯与大鼠肝脏细胞质的结合情况。在对照大鼠的肝脏细胞质中，大多数高亲和力的油酸酯结合存在于12000道尔顿蛋白质区域。在糖尿病和饥饿大鼠的肝脏细胞质中，放射性的第二个峰值出现在空体积区域。该峰值显示与一种分子量为400000的成分相关。本文提供了证据表明细胞质结合蛋白的组成发生了变化。
