Bakkari Mohammed Ali, Moni Sivakumar S, Alshammari Abdulrahman, Sultan Muhammad H, Madkhali Osama A, Almoshari Yosif, Alam Mohammad Firoz, Elmobark Mohamed Eltaib
Department of Pharmaceutics, College of Pharmacy, Jazan University, Jazan 45142, Saudi Arabia.
Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
Vaccines (Basel). 2023 Feb 16;11(2):457. doi: 10.3390/vaccines11020457.
The present study focused on demonstrating the induction of humoral and cell-mediated immunity through the establishment of a cytokine network. We hypothesized the anti-inflammatory, pro-inflammatory, and IgE antibody levels after vaccination with lyophilized recombinant HBsAg-loaded docosahexaenoic acid nanovesicles (LRPDNV), and the efficacy compared well with standard commercial recombinant hepatitis B vaccine. The cytokine network was efficiently regulated by striking a balance between pro-inflammatory cytokines IL-6, IL-8R, and IL-12 and anti-inflammatory cytokines such as IL-2, IL-4, IL-10, and IFN-γ immune response on the 14 and 30 day after primary and booster immunization. The acute phase protein CRP level was increased due to IL-6 after immunizing with LRPDNV. On the other hand, the IgE level was not significantly increased to induce any allergic reactions after immunization with LRPDNV. The study concluded that after immunizing with LRPDNV, a significant immunological response was established, implying that DHA nanovesicles have significant potential as an adjuvant method for delivering recombinant HBsAg protein. On the other hand, following immunization with LRPDNV, the IgE level was not noticeably elevated enough to cause any adverse reactions. The study concludes that a robust immune response was developed after immunizing with LRPDNV and suggests that DHA nanovesicles have much potential to deliver recombinant HBsAg protein.
本研究着重通过建立细胞因子网络来证明体液免疫和细胞介导免疫的诱导。我们假设冻干的负载重组乙肝表面抗原的二十二碳六烯酸纳米囊泡（LRPDNV）接种疫苗后抗炎、促炎和IgE抗体水平，且其效果与标准商业重组乙肝疫苗相当。在初次免疫和加强免疫后的第14天和第30天，通过在促炎细胞因子IL-6、IL-8R和IL-12与抗炎细胞因子如IL-2、IL-4、IL-10和IFN-γ免疫反应之间取得平衡，有效地调节了细胞因子网络。用LRPDNV免疫后，由于IL-6，急性期蛋白CRP水平升高。另一方面，用LRPDNV免疫后，IgE水平没有显著升高以诱导任何过敏反应。该研究得出结论，用LRPDNV免疫后，建立了显著的免疫反应，这意味着DHA纳米囊泡作为递送重组乙肝表面抗原蛋白的佐剂方法具有巨大潜力。另一方面，用LRPDNV免疫后，IgE水平没有明显升高到足以引起任何不良反应的程度。该研究得出结论，用LRPDNV免疫后产生了强烈的免疫反应，并表明DHA纳米囊泡在递送重组乙肝表面抗原蛋白方面有很大潜力。
