Division of Respirology, Rheumatology, Infectious Diseases and Neurology, Department of Internal Medicine, University of Miyazaki, Kihara 5200, Kiyotake, Miyazaki 889-1692, Japan.
Department of Clinical Laboratory, University of Miyazaki Hospital, Kihara 5200, Kiyotake, Miyazaki 889-1692, Japan.
Viruses. 2023 Feb 8;15(2):468. doi: 10.3390/v15020468.
The study aims to assess the usefulness of human T-cell leukemia virus type 1 (HTLV-1)-infected cell analysis using flow cytometry (HAS-Flow) as a monitoring method for adult T-cell leukemia (ATL) development in HTLV-1-positive patients with rheumatoid arthritis (RA) under treatment with antirheumatic therapies. A total of 13 HTLV-1-negative and 57 HTLV-1-positive RA patients participated in this study, which was used to collect clinical and laboratory data, including HAS-Flow and HTLV-1 proviral load (PVL), which were then compared between the two groups. CADM1 expression on CD4+ cells in peripheral blood (PB) was used to identify HTLV-1-infected cells. The population of CADM1+ CD4+ cells was significantly higher in HTLV-1-positive RA patients compared to HTLV-1-negative RA patients. The population of CADM1+ CD4+ cells was correlated with HTLV-1 PVL values. There were no antirheumatic therapies affecting both the expression of CADM1 on CD4+ cells and PVLs. Six HTLV-1-positive RA patients who indicated both high HTLV-1 PVL and a predominant pattern of CADM1+ CD7neg CD4+ cells in HAS-Flow can be classified as high-risk for ATL progression. HAS-Flow could be a useful method for monitoring high-risk HTLV-1-positive RA patients who are at risk of developing ATL during antirheumatic therapies.
本研究旨在评估使用流式细胞术（HAS-Flow）分析人 T 细胞白血病病毒 1（HTLV-1）感染细胞作为监测方法在接受抗风湿治疗的 HTLV-1 阳性类风湿关节炎（RA）患者中用于监测成人 T 细胞白血病（ATL）发展的有用性。共有 13 名 HTLV-1 阴性和 57 名 HTLV-1 阳性 RA 患者参与了这项研究，用于收集临床和实验室数据，包括 HAS-Flow 和 HTLV-1 前病毒载量（PVL），然后比较两组之间的数据。使用外周血（PB）中 CD4+细胞上的 CADM1 表达来识别 HTLV-1 感染细胞。与 HTLV-1 阴性 RA 患者相比，HTLV-1 阳性 RA 患者的 CADM1+ CD4+细胞群显著更高。CADM1+ CD4+细胞群与 HTLV-1 PVL 值相关。没有抗风湿治疗同时影响 CD4+细胞上 CADM1 的表达和 PVLs。六位 HTLV-1 阳性 RA 患者的 HAS-Flow 中均显示高 HTLV-1 PVL 和 CADM1+ CD7neg CD4+细胞的主要模式，可被归类为 ATL 进展的高风险。HAS-Flow 可能是一种有用的方法，可用于监测接受抗风湿治疗期间有发生 ATL 风险的高风险 HTLV-1 阳性 RA 患者。
