Centre for Ecology, Evolution and Environmental Changes (cE3c), Faculdade de Ciências da Universidade de Lisboa, 1749-016 Lisboa, Portugal.
Zoological Society of London, Institute of Zoology, Nuffield Building, Outer Circle, London NW8 7LS, UK.
Viruses. 2023 Feb 8;15(2):471. doi: 10.3390/v15020471.
Emergent infectious diseases have an increasing impact on both farmed animals and wildlife. The ability to screen for pathogens is critical for understanding host-pathogen dynamics and informing better management. is a pathogen of concern, associated with disease outbreaks worldwide, affecting a broad range of fish, amphibian, and reptile hosts, but research has been limited. The traditional screening of internal tissues, such as the liver, has been regarded as the most effective for detecting and quantifying . However, such methodology imposes several limitations from ethical and conservation standpoints. Non-lethal sampling methods of viral detection were explored by comparing the efficacy of both buccal swabbing and fin clipping. The study was conducted on two Iberian, threatened freshwater fish ( and ), and all samples were screened using qPCR. While for both methods were reliable in detecting , on , there was a significantly higher detection rate in buccal swabs than in fin tissue. This study, therefore, reports that fin clipping may yield false negatives when in small-bodied freshwater fish. Overall, buccal swabbing is found to be good as an alternative to more invasive procedures, which is of extreme relevance, particularly when dealing with a threatened species.
新发传染病对养殖动物和野生动物都有越来越大的影响。病原体筛查能力对于了解宿主-病原体动态和提供更好的管理至关重要。是一种令人关注的病原体，与世界各地的疾病爆发有关，影响广泛的鱼类、两栖类和爬行类宿主，但研究一直受到限制。传统的内部组织（如肝脏）筛查被认为是检测和定量的最有效方法。然而，从伦理和保护的角度来看，这种方法存在一些局限性。通过比较口腔拭子和鳍夹的效果，探索了病毒检测的非致死性采样方法。该研究在两种伊比利亚受威胁的淡水鱼类（和）上进行，所有样本均使用 qPCR 进行筛查。虽然对于两种方法都可以可靠地检测，但在小型淡水鱼上，口腔拭子的检测率明显高于鳍组织。因此，本研究报告称，在小体型淡水鱼中，鳍夹可能会产生假阴性结果。总的来说，口腔拭子被发现是一种很好的替代更具侵入性的程序，这在处理受威胁物种时非常重要。
