Mohammadi Ali, Danafar Hossein
Student Research Center, Zanjan University of Medical Sciences, Zanjan, Iran.
Zanjan Pharmaceutical Nanotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran.
Heliyon. 2023 Feb 14;9(2):e13740. doi: 10.1016/j.heliyon.2023.e13740. eCollection 2023 Feb.
Cancer is among the most common causes of death in the world that affects a large number of people every year. Curcumin is one of the natural anticancer therapeutics with little or no negative effects. However, due to its hydrophobic nature, poor bioavailability, limited gastrointestinal uptake, and fast metabolism, its therapeutic applications are constrained. Therefore, the Bovine Serum Albumin-Coated Copper Sulfide anoparticles (CuS@BSA) for curcumin (CUR) drug delivery were synthesized and characterized, and then curcumin release from the nanosystem. Hemotoxicity, and cytotoxicity was investigated. This study involved the one-step synthesis of CuS@BSA nanoparticles first, followed by the addition of CUR. Then the synthesized nanoparticles were characterized employing Scanning Transient Electron Microscopy (STEM), Ultraviolet-visible spectroscopy (UV-vis) and Fourier-transform infrared spectroscopy (FT-IR) techniques. The Size and surface charge (zeta potential) of synthesized nanoparticles were determined by Dynamic Light Scattering (DLS) to be 120 nm and -13 eV, respectively. The results showed that the CUR loading was around 15% and also the release pattern of CUR was dependent on pH and increased in an acidic environment. The results of the hemolysis assay showed that the synthesized nanoparticles are not hemotoxic. The investigation of the cytotoxic effects of synthesized nanoparticles on cancer cells demonstrated that CuS@BSA nanoparticles did not exhibit any toxicity and therefore are an appropriate candidate for drug delivery.
癌症是世界上最常见的死因之一，每年影响大量人群。姜黄素是一种天然抗癌治疗剂，副作用很小或没有副作用。然而，由于其疏水性、低生物利用度、有限的胃肠道吸收和快速代谢，其治疗应用受到限制。因此，合成并表征了用于姜黄素（CUR）药物递送的牛血清白蛋白包被硫化铜纳米颗粒（CuS@BSA），然后研究了姜黄素从该纳米系统中的释放、血液毒性和细胞毒性。本研究首先涉及一步合成CuS@BSA纳米颗粒，随后添加CUR。然后采用扫描瞬态电子显微镜（STEM）、紫外可见光谱（UV-vis）和傅里叶变换红外光谱（FT-IR）技术对合成的纳米颗粒进行表征。通过动态光散射（DLS）测定合成纳米颗粒的尺寸和表面电荷（zeta电位）分别为120nm和-13eV。结果表明，CUR负载量约为15%，并且CUR的释放模式取决于pH值，在酸性环境中增加。溶血试验结果表明，合成的纳米颗粒没有血液毒性。对合成纳米颗粒对癌细胞的细胞毒性研究表明，CuS@BSA纳米颗粒没有表现出任何毒性，因此是药物递送的合适候选物。
