霍乱噬菌体“κ”的紫外线灭活及光复活作用

Ultraviolet inactivation and photoreactivation of the cholera phage 'kappa'.

作者信息

Samad S A, Bhattacharyya S C, Chatterjee S N

机构信息

Biophysics Division, Saha Institute of Nuclear Physics, Calcutta, India.

出版信息

Radiat Environ Biophys. 1987;26(4):295-300. doi: 10.1007/BF01221975.

DOI:10.1007/BF01221975

PMID:3685253

Abstract

The lysogenic cholera phage, 'Kappa' is some ten to twenty folds more resistant to UV (254 nm) than are most of the T. phages of E. coli, or the cholera phage PL 163/10, or the host V. cholerae strain H218 Smr, the 37% (D37) and 10% (D10) survival doses being 255.8 J/m2 and 633.6 J/m2 respectively. The UV-irradiated 'Kappa' phages could be photoreactivated in the host V. cholerae strain H218 Smr to a maximum extent of 40%. The removal of the number of lethal hits per phage by the survival-enhancement treatment (photoreactivation) with time followed an exponential relation, the constant probability of removal of lethal hit per unit time being 2.8 x 10(-2)min-1. The UV-irradiated phages could also be Weigle reactivated in the host strain H218 Smr by a small degree, the maximum reactivation factor (ratio of survivals in UV-irradiated and non-irradiated hosts) being 1.50.

摘要

溶原性霍乱噬菌体“Kappa”对紫外线（254纳米）的抗性比大多数大肠杆菌T噬菌体、霍乱噬菌体PL 163/10或宿主霍乱弧菌菌株H218 Smr强约10至20倍，其37%（D37）和10%（D10）存活剂量分别为255.8 J/m²和633.6 J/m²。紫外线照射后的“Kappa”噬菌体在宿主霍乱弧菌菌株H218 Smr中可被光复活，最大复活程度为40%。随着时间的推移，通过存活增强处理（光复活）去除每个噬菌体的致死损伤数量遵循指数关系，单位时间内去除致死损伤的恒定概率为2.8×10⁻² min⁻¹。紫外线照射后的噬菌体在宿主菌株H218 Smr中也可被韦格尔复活，但程度较小，最大复活因子（紫外线照射宿主与未照射宿主中的存活比例）为1.50。