Department of Cardiology, Yiwu Central Hospital, Affiliated Hospital of Wenzhou Medical University, Zhejiang, China.
J Clin Ultrasound. 2023 Mar;51(3):520-529. doi: 10.1002/jcu.23433. Epub 2023 Feb 28.
Coronary heart disease (CHD) is a disorder resulting from organic and functional coronary artery stenosis (CAS), thus causing reduced oxygenated blood in the heart. miRNAs are useful biomarkers in the diagnosis of atherosclerosis, CHD, and acute coronary syndrome. Vascular endothelial growth factor (VEGF) is closely related to CHD. This study explored the correlation of miR-296 and VEGF-B expression levels in peripheral blood with CAS degree in CHD patients.
Totally 220 CHD patients were enrolled and classified into mild-(71 cases)/moderate-(81 cases)/severe-CAS (68 cases) groups, with another 80 healthy cases as controls. The serum miR-296 and VEGF-B expression levels were detected using reverse transcription quantitative polymerase chain reaction. The correlation between miR-296 and CAS-related indexes was assessed via Pearson analysis. The binding relationship of miR-296 and VEGF-B was first predicted and their correlation was further analyzed via the Pearson method. The clinical diagnostic efficacy of miR-296 or VEGF-B on CAS degree was evaluated by the receiver operating characteristic curve.
Serum miR-296 was downregulated in CHD patients and was the lowest in patients with severe-CAS. miR-296 was negatively-correlated with high-sensitivity C-reactive protein, brain natriuretic peptide, and cardiac troponin I. miR-296 targeted VEGF-B. VEGF-B was upregulated in CHD patients and inversely-related to miR-296. Low expression of miR-296 and high expression of VEGF-B both had high clinical diagnostic values on CAS degree in CHD patients. miR-296 combined with VEGF-B increased the diagnostic value on CAS.
Low expression of miR-296 combined with high expression of its target VEGF-B predicts CAS degree in CHD patients.
冠心病（CHD）是一种由有机和功能性冠状动脉狭窄（CAS）引起的疾病，导致心脏含氧血液减少。miRNA 是动脉粥样硬化、CHD 和急性冠状动脉综合征诊断的有用生物标志物。血管内皮生长因子（VEGF）与 CHD 密切相关。本研究探讨了 CHD 患者外周血 miR-296 和 VEGF-B 表达水平与 CAS 程度的相关性。
共纳入 220 例 CHD 患者，分为轻度（71 例）/中度（81 例）/重度 CAS（68 例）组，另选 80 例健康者作为对照组。采用逆转录定量聚合酶链反应检测血清 miR-296 和 VEGF-B 表达水平。通过 Pearson 分析评估 miR-296 与 CAS 相关指标的相关性。首先预测 miR-296 与 VEGF-B 的结合关系，然后通过 Pearson 方法分析它们的相关性。通过受试者工作特征曲线评估 miR-296 或 VEGF-B 对 CAS 程度的临床诊断效能。
CHD 患者血清 miR-296 下调，重度 CAS 患者最低。miR-296 与高敏 C 反应蛋白、脑钠肽和心肌肌钙蛋白 I 呈负相关。miR-296 靶向 VEGF-B。CHD 患者 VEGF-B 上调，与 miR-296 呈负相关。miR-296 低表达和 VEGF-B 高表达对 CHD 患者的 CAS 程度均具有较高的临床诊断价值。miR-296 与 VEGF-B 联合检测可提高对 CAS 的诊断价值。
miR-296 低表达与靶基因 VEGF-B 高表达共同预测 CHD 患者的 CAS 程度。
