Department of Nosocomial Infection Management, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Edong Healthcare Group, Huangshi, China.
Department of Thoracic and Cardiovascular Surgery, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Edong Healthcare Group, Huangshi, China.
J Clin Lab Anal. 2022 Feb;36(2):e24202. doi: 10.1002/jcla.24202. Epub 2022 Jan 8.
Long noncoding RNA GAS5 (lnc-GAS5) and its target microRNA-21 (miR-21) regulate blood lipid, macrophages, Th cells, vascular smooth muscle cells to participate in atherosclerosis, and related coronary heart disease (CHD). The study aimed to further explore the linkage of their circulating expressions with common biochemical indexes, stenosis severity and inflammatory cytokines in CHD patients.
Ninety-eight CHD patients and 100 controls confirmed by coronary angiography were enrolled. Plasma samples were collected for lnc-GAS5 and miR-21 detection by reverse transcription-quantitative polymerase chain reaction and inflammatory cytokines determination by enzyme-linked immunosorbent assay.
Lnc-GAS5 was increased in CHD patients compared with controls (2.270 (interquartile range [IQR]: 1.676-3.389) vs. 0.999 ([IQR: 0.602-1.409], p < 0.001), whereas miR-21 showed opposite tread (0.442 [IQR: 0.318-0.698] vs. 0.997 [IQR: 0.774-1.368], p < 0.001). In aspect of their intercorrelation, lnc-GAS5 negatively linked with miR-21 in CHD patients (p < 0.001) instead of controls (p = 0.211). Interestingly, among the common biochemical indexes, lnc-GAS5 related to decreased high-density lipoprotein cholesterol (p = 0.008) and increased C-reactive protein (CRP) (p < 0.001), while miR-21 correlated with lower total cholesterol (p = 0.024) and CRP (p < 0.001) in CHD patients. As stenosis degree, lnc-GAS5 positively correlated with Gensini score (p < 0.001), but miR-21 exhibited negative association (p = 0.003) in CHD patients. In terms of inflammatory cytokines, lnc-GAS5 positively related to tumor necrosis factor α (TNF-α) and interleukin (IL)-17A, while miR-21 negatively linked with TNF-α, IL-1β, IL-6, and IL-17 in CHD patients (all p < 0.05).
Circulating lnc-GAS5 and its target miR-21 exhibit potency to serve as biomarkers for CHD management.
长链非编码 RNA GAS5（lnc-GAS5）及其靶 microRNA-21（miR-21）可调节血脂、巨噬细胞、Th 细胞、血管平滑肌细胞，参与动脉粥样硬化及相关冠心病（CHD）的发生。本研究旨在进一步探讨其循环表达与 CHD 患者常见生化指标、狭窄严重程度和炎症因子之间的关联。
通过冠状动脉造影，共纳入 98 例 CHD 患者和 100 例对照者。采集血浆样本，采用逆转录定量聚合酶链反应检测 lnc-GAS5 和 miR-21，采用酶联免疫吸附试验检测炎症因子。
与对照组相比，CHD 患者的 lnc-GAS5 升高（2.270（四分位距 [IQR]：1.676-3.389）比 0.999（IQR：0.602-1.409），p<0.001），而 miR-21 则呈相反趋势（0.442（IQR：0.318-0.698）比 0.997（IQR：0.774-1.368），p<0.001）。lnc-GAS5 与 miR-21 在 CHD 患者中呈负相关（p<0.001），而在对照组中无相关性（p=0.211）。有趣的是，在常见的生化指标中，lnc-GAS5 与高密度脂蛋白胆固醇降低（p=0.008）和 C 反应蛋白（CRP）升高（p<0.001）有关，而 miR-21 与总胆固醇降低（p=0.024）和 CRP 降低（p<0.001）有关。随着狭窄程度的增加，lnc-GAS5 与 Gensini 评分呈正相关（p<0.001），而 miR-21 呈负相关（p=0.003）。在炎症因子方面，lnc-GAS5 与肿瘤坏死因子-α（TNF-α）和白细胞介素（IL）-17A 呈正相关，而 miR-21 与 TNF-α、IL-1β、IL-6 和 IL-17 呈负相关（均 p<0.05）。
循环 lnc-GAS5 及其靶 miR-21 可作为 CHD 管理的潜在生物标志物。
