Department of Pathology and Lab Medicine, All India Institute of Medical Sciences (AIIMS), Jodhpur, India.
Department of General Surgery, All India Institute of Medical Sciences (AIIMS), Jodhpur, India.
Asian Pac J Cancer Prev. 2023 Feb 1;24(2):509-515. doi: 10.31557/APJCP.2023.24.2.509.
Mammaglobin and GCDFP-15 are traditional immunohistochemistry (IHC) markers utilized to recognize metastasis of breast carcinoma in an unknown primary. GATA-3 is increasingly being used as a marker of primary breast origin. This study was done to evaluate and compare GATA-3 with GCDFP-15 and Mammaglobin in invasive primary including metastatic and triple negative breast carcinomas.
Immunohistochemistry for GATA-3, GCDFP-15 and Mammaglobin was applied on 100 cases of primary breast carcinomas, including 20 triple negative cases and 30 cases of metastatic breast carcinomas. Staining scores were given for each marker by multiplying the percentage of positive tumor cells by the intensity of staining (1+, 2+ or 3+), with scores ranging from 0 to 300. Staining score of 1 or more was considered positive.
GATA-3 was expressed in 92% of primary, 80% of metastatic and 60% of triple negative breast carcinomas, with an average staining score of 270. Mammaglobin was expressed in 68% of primary, 56.6% of metastatic and 25% of triple negative breast carcinomas, with an average staining score of 180. GCDFP-15 was expressed in 48% of primary, 26.6% of metastatic and 05% of breast carcinomas, with an average staining score of 60. GATA-3 demonstrated to have higher staining score (average of 270) than other two markers in maximum number of cases.
GATA-3 has a higher sensitivity and increased staining scores in primary breast carcinomas, metastatic breast carcinomas as well as in triple negative breast carcinomas.
在未知原发性肿瘤中，Mammaglobin 和 GCDFP-15 是传统免疫组织化学（IHC）标志物，用于识别乳腺癌转移。GATA-3 越来越多地被用作原发性乳腺癌的标志物。本研究旨在评估和比较 GATA-3 与 GCDFP-15 和 Mammaglobin 在浸润性原发性乳腺癌（包括转移性和三阴性乳腺癌）中的应用。
对 100 例原发性乳腺癌病例进行 GATA-3、GCDFP-15 和 Mammaglobin 的免疫组织化学染色，包括 20 例三阴性病例和 30 例转移性乳腺癌病例。每个标志物的染色评分通过将阳性肿瘤细胞的百分比乘以染色强度（1+、2+或 3+）相乘得到，评分范围为 0 至 300。评分 1 或以上为阳性。
GATA-3 在 92%的原发性、80%的转移性和 60%的三阴性乳腺癌中表达，平均染色评分 270。Mammaglobin 在 68%的原发性、56.6%的转移性和 25%的三阴性乳腺癌中表达，平均染色评分 180。GCDFP-15 在 48%的原发性、26.6%的转移性和 05%的乳腺癌中表达，平均染色评分 60。GATA-3 在最大数量的病例中表现出比其他两种标志物更高的染色评分（平均 270）。
GATA-3 在原发性乳腺癌、转移性乳腺癌以及三阴性乳腺癌中具有更高的敏感性和增加的染色评分。
