饲料中十种单端孢霉烯族化合物同时定量的实验室间研究。
Inter-laboratory study on simultaneous quantification of ten trichothecenes in feed.
机构信息
Food and Agricultural Materials Inspection Center, Fertilizer and Feed Inspection Department, Saitama Shintoshin National Government Building, Kensato Building, Shintoshin 2-1, Chuo-ku, Saitama-shi, 330-9731, Saitama, Japan.
出版信息
Mycotoxin Res. 2023 May;39(2):95-108. doi: 10.1007/s12550-023-00476-4. Epub 2023 Feb 28.
An inter-laboratory study was performed in eight laboratories to evaluate the simultaneous quantification method for HT-2 toxin (HT-2), T-2 toxin (T-2), diacetoxyscirpenol (DAS), neosolaniol (NES), 3-acetyldeoxynivalenol (3-AcDON), 15-acetyldeoxynivalenol (15-AcDON), deoxynivalenol (DON), deoxynivalenol-3-glucoside (D3G), nivalenol (NIV), and fusarenon-X (FUS-X) in feed. The mycotoxins in the samples were extracted with hydrous acetonitrile, purified using a multifunctional column (InertSep VRA-3) and a phospholipid removal column (Hybrid SPE-Phospholipid), and then quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with atmospheric pressure chemical ionisation mode. The mean recovery, repeatability, reproducibility, and Horwitz ratio from the inter-laboratory validation study were 99.8-109%, 3.1-9.8%, 4.3-9.8%, and 0.19-0.45, respectively, for type A trichothecenes (HT-2, T-2, DAS, and NES). Those values for type B trichothecenes (3-AcDON, 15-AcDON, DON, NIV, and FUS-X) were 89.9-116%, 3.4-9.1%, 5.6-14%, and 0.25-0.70, and the values for modified mycotoxin (D3G) were 78.2-96.7%, 3.5-6.4%, and 13-22%, respectively.
在八个实验室进行了一项实验室间研究，以评估同时定量测定饲料中 HT-2 毒素 (HT-2)、T-2 毒素 (T-2)、二乙酰氧基麦角甾醇 (DAS)、新茄病镰刀菌烯醇 (NES)、3-乙酰脱氧雪腐镰刀菌烯醇 (3-AcDON)、15-乙酰脱氧雪腐镰刀菌烯醇 (15-AcDON)、脱氧雪腐镰刀菌烯醇 (DON)、脱氧雪腐镰刀菌烯醇-3-葡萄糖苷 (D3G)、伏马菌素 (NIV) 和镰刀菌烯酮-X (FUS-X) 的方法。样品中的真菌毒素用含水乙腈提取，用多功能柱（InertSep VRA-3）和磷脂去除柱（Hybrid SPE-Phospholipid）纯化，然后用大气压化学电离模式的液相色谱-串联质谱法（LC-MS/MS）定量。来自实验室间验证研究的平均回收率、重复性、再现性和霍维茨比分别为 99.8-109%、3.1-9.8%、4.3-9.8%和 0.19-0.45，用于 A 型单端孢霉烯族化合物（HT-2、T-2、DAS 和 NES）。B 型单端孢霉烯族化合物（3-AcDON、15-AcDON、DON、NIV 和 FUS-X）的这些值分别为 89.9-116%、3.4-9.1%、5.6-14%和 0.25-0.70，修饰真菌毒素（D3G）的这些值分别为 78.2-96.7%、3.5-6.4%和 13-22%。
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