Institute of Neuropathology, University of Zurich, 8091 Zurich, Switzerland.
Fluidic Analytics, Unit A, The Paddocks Business Centre, Cherry Hinton Road, Cambridge CB1 8DH, UK.
STAR Protoc. 2023 Mar 17;4(1):102095. doi: 10.1016/j.xpro.2023.102095. Epub 2023 Feb 13.
Conventional methods of measuring affinity are limited by artificial immobilization, large sample volumes, and homogeneous solutions. This protocol describes microfluidic antibody affinity profiling on complex human samples in solution to obtain a fingerprint reflecting both affinity and active concentration of the target protein. To illustrate the protocol, we analyze the antibody response in SARS-CoV-2 omicron-naïve samples against different SARS-CoV-2 variants of concern. However, the protocol and the technology are amenable to a broad spectrum of biomedical questions. For complete details on the use and execution of this protocol, please refer to Emmenegger et al. (2022), Schneider et al. (2022), and Fiedler et al. (2022)..
传统的亲和力测量方法受到人为固定化、大样本体积和均相溶液的限制。本方案描述了在溶液中对复杂的人类样本进行微流控抗体亲和力分析，以获得反映目标蛋白亲和力和有效浓度的指纹图谱。为了说明该方案，我们分析了针对不同关注的 SARS-CoV-2 变体的 SARS-CoV-2 奥密克戎初免样本中的抗体反应。然而，该方案和技术适用于广泛的生物医学问题。有关该方案使用和执行的完整详细信息，请参阅 Emmenegger 等人（2022 年）、Schneider 等人（2022 年）和 Fiedler 等人（2022 年）。
