Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China.
College of Life Science, Nankai University, Tianjin 300071, China.
STAR Protoc. 2022 Feb 28;3(2):101238. doi: 10.1016/j.xpro.2022.101238. eCollection 2022 Jun 17.
The immunogenicity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteome is largely unknown. Here we describe a protocol for analyzing sera samples with SARS-CoV-2 proteome microarray. The proteins were expressed by either expression system or eukaryotic cell expression systems and obtained by affinity purification. The protocol includes microarray fabricating and sera profiling, which will be used to build an antibody response landscape for IgG and IgM. The protocol may help to facilitate a deeper understanding of immunity related to SARS-CoV-2. For complete details on the use and execution of this protocol, please refer to Li et al. (2021c).
严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）蛋白质组的免疫原性在很大程度上是未知的。在这里，我们描述了一种使用 SARS-CoV-2 蛋白质组微阵列分析血清样本的方案。这些蛋白质通过表达系统或真核细胞表达系统表达，并通过亲和纯化获得。该方案包括微阵列的制作和血清分析，这将用于构建 IgG 和 IgM 的抗体反应图谱。该方案可能有助于促进对 SARS-CoV-2 相关免疫的更深入了解。有关该方案使用和执行的完整详细信息，请参考 Li 等人（2021c）。
