整合 SNP 基因分型和 16S rRNA 扩增子测序技术，以鉴定鸡的可遗传肠道微生物。
Integration of SNP genotyping and 16S rRNA amplicon sequencing to identify heritable gut microbes in chickens.
机构信息
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, PR China.
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, PR China.
出版信息
STAR Protoc. 2023 Mar 17;4(1):102071. doi: 10.1016/j.xpro.2023.102071. Epub 2023 Jan 27.
The effect of host genetics on the gut microbiota is not fully understood. Here, we introduce a protocol that describes the steps necessary to analyze the SNP genotyping and amplicon sequencing data to identify heritable microbes in chicken gut. We apply this protocol to infer the cecal heritable taxa and their associated SNPs in chicken genome sequence. This will be beneficial for the identification of gut microbes that are influenced by host genetics in both humans and animals. For complete details on the use and execution of this protocol, please refer to Feng et al. (2022)..
宿主遗传学对肠道微生物群的影响尚未完全阐明。在这里，我们介绍了一种方案，描述了分析 SNP 基因分型和扩增子测序数据以鉴定鸡肠道中可遗传微生物所需的步骤。我们应用此方案来推断鸡基因组序列中盲肠可遗传分类群及其相关 SNP。这将有助于鉴定受宿主遗传影响的肠道微生物，无论是在人类还是动物中。有关该方案的使用和执行的完整详细信息，请参阅 Feng 等人（2022 年）。
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