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优化方案用于定量检测小鼠组织和培养细胞中线粒体中非血红素和血红素铁含量。

Optimized protocol for quantification of mitochondrial non-heme and heme iron content in mouse tissues and cultured cells.

机构信息

Feinberg Cardiovascular Research Institute, Northwestern University School of Medicine, Chicago, IL, USA; Department of Cellular Physiology and Signal Transduction, Sapporo Medical University School of Medicine, Sapporo, Japan.

Feinberg Cardiovascular Research Institute, Northwestern University School of Medicine, Chicago, IL, USA.

出版信息

STAR Protoc. 2023 Mar 17;4(1):102064. doi: 10.1016/j.xpro.2023.102064. Epub 2023 Jan 24.

DOI:10.1016/j.xpro.2023.102064
PMID:36853672
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9881402/
Abstract

Impaired mitochondrial iron metabolism is associated with aging and a variety of diseases, and there is a growing need to accurately quantify mitochondrial iron levels. This protocol provides an optimized method for evaluating non-heme and heme iron in mitochondrial and cytosolic fractions of tissues and cultured cells. Our protocol consists of three steps: sample fractionation, non-heme iron measurement, and heme iron measurement. For complete details on the use and execution of this protocol, please refer to Sato et al. (2022)..

摘要

线粒体铁代谢受损与衰老和多种疾病有关，因此需要更准确地定量线粒体铁水平。本方案提供了一种优化的方法，用于评估组织和培养细胞的线粒体和胞质部分中的非血红素铁和血红素铁。我们的方案包括三个步骤：样品分级分离、非血红素铁测量和血红素铁测量。有关该方案使用和执行的详细信息，请参阅 Sato 等人（2022 年）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa13/9881402/8a1ce748a5a8/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa13/9881402/0721b2960811/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa13/9881402/2c2263e82032/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa13/9881402/560f8a7f9030/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa13/9881402/154f8c25c133/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa13/9881402/8a1ce748a5a8/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa13/9881402/0721b2960811/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa13/9881402/2c2263e82032/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa13/9881402/560f8a7f9030/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa13/9881402/154f8c25c133/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa13/9881402/8a1ce748a5a8/gr4.jpg

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