Department of Surgery, Division of Surgical Oncology, Cancer Research Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02214, USA.
STAR Protoc. 2021 Apr 22;2(2):100491. doi: 10.1016/j.xpro.2021.100491. eCollection 2021 Jun 18.
Labile heme is present in the cells at very low concentrations, either unbound or loosely bound to molecules, and accessible for signaling as alarmin. Our recent work suggests that extracellular heme can be taken up and detected in the nuclei of cancer cells. Here, we describe the detailed protocol for detection of labile and total heme in prostate cancer cells and its measurement in subcellular compartments . The protocol can be adapted to be used for other cell types. For complete details on the use and execution of this protocol, please refer to Canesin et al. (2020).
不稳定的血红素以非常低的浓度存在于细胞中，要么未结合，要么与分子松散结合，并可作为警报素进行信号传递。我们最近的工作表明，细胞外血红素可以被摄取并在癌细胞的核内检测到。在这里，我们描述了检测前列腺癌细胞中不稳定和总血红素及其在亚细胞区室中测量的详细方案。该方案可以适用于其他细胞类型。有关此方案的使用和执行的完整详细信息，请参阅 Canesin 等人。（2020 年）。
