评估 SARS-CoV-2 刺突假病毒样颗粒诱导人巨噬细胞炎症反应的方案。
Protocol to evaluate the inflammatory response in human macrophages induced by SARS-CoV-2 spike-pseudotyped VLPs.
机构信息
Department of Medical Microbiology, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R3E 0J9, Canada.
Department of Medical Microbiology, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R3E 0J9, Canada.
出版信息
STAR Protoc. 2023 Mar 17;4(1):102083. doi: 10.1016/j.xpro.2023.102083. Epub 2023 Jan 17.
The excessive release of pro-inflammatory cytokines in COVID-19 patients is deleterious to organs. The contribution of SARS-CoV-2 spike protein (S) to the inflammatory response is essential to understand its pathogenesis and virulence. Here, we present a protocol to produce and characterize HIV- and SARS-CoV-2-based virus-like particles and then evaluate the inflammatory cytokines' protein and mRNA levels produced in human macrophages by S of SARS-CoV-2 original strain and Delta variant. This protocol is applicable in evaluating S from different emerging variants. For complete details on the use and execution of this protocol, please refer to Ao et al. (2022)..
在 COVID-19 患者中，促炎细胞因子的过度释放对器官是有害的。了解 SARS-CoV-2 刺突蛋白（S）对炎症反应的贡献对于理解其发病机制和毒力至关重要。在这里，我们提出了一种生产和表征 HIV-和 SARS-CoV-2 基于病毒样颗粒的方案，然后评估 SARS-CoV-2 原始株和 Delta 变异株的 S 在人巨噬细胞中产生的炎症细胞因子的蛋白和 mRNA 水平。该方案适用于评估来自不同新兴变异株的 S。有关该方案使用和执行的完整详细信息，请参阅 Ao 等人。（2022）。
Zotero 插件