Division of Endocrinology and Metabolism, Department of Internal Medicine III, Medical University of Vienna, 1090 Vienna, Austria.
Division of Endocrinology and Metabolism, Department of Internal Medicine III, Medical University of Vienna, 1090 Vienna, Austria.
STAR Protoc. 2023 Mar 17;4(1):102089. doi: 10.1016/j.xpro.2023.102089. Epub 2023 Feb 9.
Tracer techniques to assess very-low-density lipoprotein (VLDL) secretion in humans are expensive, are time consuming, and require mathematical models to estimate VLDL kinetics. Here, we describe an alternative, time- and cost-efficient protocol to directly determine VLDL secretion with an intravenous (i.v.) lipid emulsion test that does not require tracers and compartmental modeling. We describe steps for intralipid infusion, blood sampling, and removal of intralipid from plasma samples, followed by density gradient ultracentrifugation to isolate VLDL fraction and measure the secretion rate. For complete details on the use and execution of this protocol, please refer to Bjorkegren et al. (1996), Al-Shayji et al. (2007), and Metz et al. (2022)..
用于评估人体极低密度脂蛋白 (VLDL) 分泌的示踪技术既昂贵又耗时，并且需要数学模型来估计 VLDL 动力学。在这里，我们描述了一种替代方法，即使用静脉内 (i.v.) 脂肪乳剂测试直接确定 VLDL 分泌的、具有时间和成本效益的协议，该协议不需要示踪剂和隔室建模。我们描述了脂肪乳剂输注、采血以及从血浆样品中去除脂肪乳剂的步骤，然后进行密度梯度超速离心分离 VLDL 级分并测量分泌率。有关此协议的使用和执行的完整详细信息，请参阅 Bjorkegren 等人（1996 年）、Al-Shayji 等人（2007 年）和 Metz 等人（2022 年）。
