Qu Lulu, Han Jing, Huang Yi, Yang Guohai, Liu Weijie, Long Zhouyang, Gu Yingqiu, Zhang Qingming, Gao Ming, Dong Xiaochen
School of Chemistry & Materials Science, Jiangsu Normal University, Xuzhou 221116, Jiangsu, China.
School of Life Science, Jiangsu Normal University, Xuzhou 221116, Jiangsu, China.
ACS Appl Bio Mater. 2023 Mar 20;6(3):1272-1282. doi: 10.1021/acsabm.3c00008. Epub 2023 Feb 28.
The emergence of nanozymes provides a potential method for combating multidrug-resistant bacteria resulted from the abuse of antibiotics. However, in nanozyme-catalyzed systems, few studies have addressed the actual hydrogen peroxide (HO) level involved in sterilization. Herein, we designed a high-efficiency peroxidase-mimicking nanozyme with surface-enhanced Raman scattering (SERS) property by assembling gold nanoparticles on single-layer Cu-CN (AuNP-Cu-CN). The nanozyme effectively converts the low-active Raman reporter 3,3',5,5'-tetramethylbenzidine (TMB) into its oxidized form with HO, resulting in SERS signal changes, thereby achieving highly sensitive quantification of HO with limit of detection as low as 0.60 μM. More importantly, the nanozyme can specifically catalyze HO into antibacterial hydroxyl radicals. and evaluations demonstrate the remarkable antibacterial efficacy of the nanozyme/HO combination against (up to 99.9%), which could promote wound healing in mice and allow point-of-care monitoring the amount of HO participated in effective sterilization. This study not only displays great potential in combining multiple functionalities of nanomaterials for versatile bioassays but also provides a promising approach to design nanozymes for biomedical and catalytic applications.
纳米酶的出现为对抗因抗生素滥用导致的多重耐药细菌提供了一种潜在方法。然而，在纳米酶催化体系中，很少有研究涉及杀菌过程中实际的过氧化氢（HO）水平。在此，我们通过在单层Cu-CN（AuNP-Cu-CN）上组装金纳米颗粒，设计了一种具有表面增强拉曼散射（SERS）特性的高效过氧化物酶模拟纳米酶。该纳米酶能有效地将低活性拉曼报告分子3,3',5,5'-四甲基联苯胺（TMB）与HO转化为其氧化形式，导致SERS信号变化，从而实现对HO的高灵敏度定量，检测限低至0.60 μM。更重要的是，该纳米酶能特异性地将HO催化生成抗菌羟基自由基。评估表明，纳米酶/HO组合对（高达99.9%）具有显著的抗菌效果，可促进小鼠伤口愈合，并能在床旁监测参与有效杀菌的HO量。本研究不仅在结合纳米材料的多种功能用于多功能生物测定方面显示出巨大潜力，还为设计用于生物医学和催化应用的纳米酶提供了一种有前景的方法。
