Xu Yang, Wang Qian, Wang Xiangxiu, Xiang Xiaona, Peng Jialei, Zhang Jiangyin, He Hongchen
Rehabilitation Medicine Centre, West China Hospital of Sichuan University, Chengdu 610041, P. R. China.
West China School of Medicine, Sichuan University, Chengdu 610041, P. R. China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2023 Feb 25;40(1):95-102. doi: 10.7507/1001-5515.202209053.
The study aims to explore the effect of mesenchymal stem cells-derived exosomes (MSCs-Exo) on staurosporine (STS)-induced chondrocyte apoptosis before and after exposure to pulsed electromagnetic field (PEMF) at different frequencies. The AMSCs were extracted from the epididymal fat of healthy rats before and after exposure to the PEMF at 1 mT amplitude and a frequency of 15, 45, and 75 Hz, respectively, in an incubator. MSCs-Exo was extracted and identified. Exosomes were labeled with DiO fluorescent dye, and then co-cultured with STS-induced chondrocytes for 24 h. Cellular uptake of MSC-Exo, apoptosis, and the protein and mRNA expression of aggrecan, caspase-3 and collagenⅡA in chondrocytes were observed. The study demonstrated that the exposure of 75 Hz PEMF was superior to 15 and 45 Hz PEMF in enhancing the effect of exosomes in alleviating chondrocyte apoptosis and promoting cell matrix synthesis. This study lays a foundation for the regulatory mechanism of PEMF stimulation on MSCs-Exo in inhibiting chondrocyte apoptosis, and opens up a new direction for the prevention and treatment of osteoarthritis.
本研究旨在探讨间充质干细胞来源的外泌体（MSCs-Exo）在不同频率脉冲电磁场（PEMF）作用前后对星形孢菌素（STS）诱导的软骨细胞凋亡的影响。分别在培养箱中以1 mT的振幅、15、45和75 Hz的频率对健康大鼠附睾脂肪进行PEMF照射，照射前后提取脂肪间充质干细胞（AMSCs）。提取并鉴定MSCs-Exo。用DiO荧光染料标记外泌体，然后与STS诱导的软骨细胞共培养24 h。观察软骨细胞对MSC-Exo的摄取、凋亡情况以及软骨细胞中聚集蛋白聚糖、半胱天冬酶-3和Ⅱ型胶原A的蛋白和mRNA表达。研究表明，75 Hz的PEMF照射在增强外泌体减轻软骨细胞凋亡和促进细胞基质合成的作用方面优于15 Hz和45 Hz的PEMF照射。本研究为PEMF刺激MSCs-Exo抑制软骨细胞凋亡的调控机制奠定了基础，并为骨关节炎的防治开辟了新方向。
