Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt.
Clinical Laboratory Diagnosis, Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt.
Sci Rep. 2023 Feb 28;13(1):3421. doi: 10.1038/s41598-023-30543-w.
This study investigated the effect of the silver nanoparticles (AgNPs) and platelet-rich fibrin (PRF) in the healing of the severed superficial digital flexor tendon in donkeys (SDFT). Twenty-seven adult donkeys were used in the study. The animals were divided into three equal groups. The first group (control group) in which the severed SDFT was sutured without the addition of any adjuvant. In the second group, there was a suture of severed SDFT with the addition of 1 ml of 1 mM silver nanoparticles (AgNPs group). The third group was subjected to the cutting of SDFT and then the addition of PRF after its suture. Each group of animals was divided into three equal subgroups that were examined after 1, 2, and 3 months. Each group of animals was clinically evaluated by assessing lameness. Gross and microscopic examinations of the healed tendons were performed after 1, 2, and 3 months of surgery. In comparison to the control group, the lameness degree decreased in the PRF and AgNPs groups, particularly in the third month after surgery. Furthermore, the lameness decreased significantly after the 3rd month relative to the 1st-month lameness in the AgNPs group. Interestingly, it was found that the PRF and AgNPs enhanced cell alignment and collagen deposition at the site of tendon injury, particularly among third-month subgroups. Therefore, it could be concluded that the PRF and AgNPs are effective materials for enhancing SDFT healing in donkeys.
本研究旨在探讨纳米银颗粒（AgNPs）和富血小板纤维蛋白（PRF）在修复切断的驴（SDFT）浅表屈肌腱中的作用。本研究共使用了 27 匹成年驴。将这些动物随机分为三组。第一组（对照组），即未添加任何辅料的切断 SDFT 缝合组；第二组，即切断 SDFT 缝合后添加 1ml1mM 纳米银颗粒（AgNPs 组）；第三组，即切断 SDFT 后添加 PRF 组。每组动物又随机分为三个亚组，分别在术后 1、2 和 3 个月进行检查。每组动物均通过跛行评估进行临床评估。术后 1、2 和 3 个月对愈合后的肌腱进行大体和显微镜检查。与对照组相比，PRF 和 AgNPs 组的跛行程度降低，特别是在术后第三个月。此外，与术后第一个月相比，AgNPs 组在第三个月跛行程度显著降低。有趣的是，研究发现 PRF 和 AgNPs 可增强损伤肌腱部位的细胞排列和胶原沉积，尤其是在第三个月的亚组中。因此，可得出结论，PRF 和 AgNPs 是增强驴 SDFT 愈合的有效材料。
