Department of Burns & Wound Care Center, Second Affiliated Hospital of Medical College, Zhejiang University, Hangzhou, 310009, China.
Sci Rep. 2017 Sep 5;7(1):10489. doi: 10.1038/s41598-017-10481-0.
Treatment of full-thickness skin defects poses significant clinical challenges including risk of infection and severe scaring. Silver nanoparticle (NAg), an effective antimicrobial agent, has provided a promising therapeutic method for burn wounds. However, the detailed mechanism remains unknown. Hence, we constructed a metallic nanosilver particles-collagen/chitosan hybrid scaffold (NAg-CCS) and investigated its potential effects on wound healing. In vitro scratch assay, immunofluorescence staining and antibacterial activity of the scaffold were all studied. In vivo NAg-CCS was applied in full-thickness skin defects in Sprague-Dawley (SD) rats and the therapeutic effects of treatment were evaluated. The results showed that NAg at a concentration of 10 ppm accelerated the migration of fibroblasts with an increase in expression of α-smooth muscle actin (α-SMA). Furthermore, in vivo studies showed increased levels of pro-inflammatory and scar-related factors as well as α-SMA, while markers for macrophage activation were up-regulated. On day 60 post transplantation of ultra-thin skin graft, the regenerated skin by NAg-CCS had a similar structure to normal skin. In summary, we demonstrated that NAg-CCS was bactericidal, anti-inflammatory and promoted wound healing potentially by regulating fibroblast migration and macrophage activation, making it an ideal dermal substitute for wound regeneration.
全层皮肤缺损的治疗存在重大的临床挑战，包括感染和严重瘢痕的风险。纳米银（NAg）作为一种有效的抗菌剂，为烧伤创面提供了一种有前途的治疗方法。然而，其详细机制尚不清楚。因此，我们构建了一种金属纳米银颗粒-胶原/壳聚糖杂化支架（NAg-CCS），并研究了其在伤口愈合中的潜在作用。研究了支架的体外划痕试验、免疫荧光染色和抗菌活性。将 NAg-CCS 应用于 Sprague-Dawley（SD）大鼠的全层皮肤缺损，并评估其治疗效果。结果表明，浓度为 10ppm 的 NAg 可促进成纤维细胞的迁移，增加α-平滑肌肌动蛋白（α-SMA）的表达。此外，体内研究表明，促炎和瘢痕相关因子以及α-SMA 的水平升高，而巨噬细胞激活的标志物上调。在超薄皮片移植后 60 天，NAg-CCS 再生的皮肤具有与正常皮肤相似的结构。总之，我们证明了 NAg-CCS 具有杀菌、抗炎作用，并通过调节成纤维细胞迁移和巨噬细胞激活促进伤口愈合，使其成为一种理想的用于创面再生的真皮替代物。
