College of Life Science and Technology, College of Agronomy, State Key Laboratory of Aridland Crop Science, Gansu Provincial Key Lab of Good Agricultural Production for Traditional Chinese Medicine, Gansu Provincial Engineering Research Centre for Medical Plant Cultivation and Breeding, Gansu Agricultural University, Lanzhou, 730070, China.
Pharmacy Department, Gansu University of Chinese Medicine, Dingxi, 743000, China.
Sci Rep. 2023 Feb 28;13(1):3388. doi: 10.1038/s41598-023-30549-4.
Angelica sinensis (Oliv.) Diels is an important Chinese medicinal plant. A. sinensis seedlings are grown on an undisturbed alpine meadow soil to ensure the high-quality seedlings, but these soils are disappearing year after year. Thus, selecting a suitable bed soil for A. sinensis seedlings could ensure their long-term sustainability. Using HiSeq sequencing of 16S and 18S marker genes, we investigated the rhizosphere bacterial and fungal microbiotas of the seedlings grown in wheat, astragalus, potato, and angelica-cultivated soils at a geo-authentic habitat. Co-occurrence network analysis, canonical correspondence analysis, Mantel test, and Envfit test were used to examine the relationship between the microbiotas and the surrounding factors. Astragalus-cultivated soils exhibited the following properties: the highest plant weight, the highest neighborhood connectivity in the bacterial network, the highest ratio of positive/negative relationship in both bacterial and fungal networks, the highest relative abundance of the arbuscular mycorrhizal fungi and the ectomycorrhizal fungi, the lowest relative abundance of Rhizoctonia solani, the suitable soil pH, and the close relationship between the rhizosphere microbiotas and the ecological factors. Moreover, each growth stage has its own major drivers in all crop-cultivated soils. Climate temperature and soil pH at 56 days after planting, precipitation at 98 days, and plant weight as well as microbial biomass C and N at 129 days were the major drivers of the bacterial and fungal microbiotas. Overall, the astragalus-cultivated soil was a suitable bed soil for nurturing A. sinensis seedlings to replace the undisturbed alpine meadow soils.
当归（Angelica sinensis (Oliv.) Diels）是一种重要的药用植物。为了确保种苗的高质量，采用未受干扰的高山草甸土壤来培育当归种苗，但这些土壤正逐年消失。因此，选择适宜的当归种苗床土对于其长期可持续发展至关重要。本研究采用 16S 和 18S 标记基因的 HiSeq 测序，对在真实生境下，生长于小麦、黄芪、土豆和当归栽培土壤中的当归种苗的根际细菌和真菌微生物群落进行了调查。利用共现网络分析、典范对应分析、Mantel 检验和 Envfit 检验，研究了微生物群落与周围环境因素的关系。黄芪栽培土壤具有以下特点：植株生物量最高、细菌网络中节点连接度最高、细菌和真菌网络中正/负关系比例最高、丛枝菌根真菌和外生菌根真菌相对丰度最高、立枯丝核菌相对丰度最低、土壤 pH 适宜，且根际微生物群落与生态因子关系密切。此外，在所有作物栽培土壤中，每个生长阶段都有其主要驱动因素。在种植后 56 天，气候温度和土壤 pH、98 天的降水、129 天的植株生物量以及微生物生物量 C 和 N 是影响细菌和真菌微生物群落的主要因素。总的来说，黄芪栽培土壤是培育当归种苗的适宜床土，可以替代未受干扰的高山草甸土壤。
