An Zhigang, Guo Fengxia, Chen Yuan, Bai Gang, Chen Zhengjun
College of Life Science and Technology, College of Agronomy, Gansu Provincial Key Lab of Good Agricultural Production for Traditional Chinese Medicine, Gansu Provincial Engineering Research Centre for Medical Plant Cultivation and Breeding, Provincial Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, China.
Pharmacy Department, Gansu University of Chinese Medicine, Dingxi, China.
PeerJ. 2020 Mar 26;8:e8541. doi: 10.7717/peerj.8541. eCollection 2020.
seedlings are grown in alpine uncultivated meadow soil with rainfed agroecosystems to ensure the quality of after seedling transplantation. The aim was to investigate the rhizosphere bacterial and fungal communities during the growth stages of seedlings.
The bacterial and fungal communities were investigated by HiSeq sequencing of 16S and 18S rDNA, respectively.
Proteobacteria and Bacteroidetes were bacterial dominant phyla throughout growth stages. Fungal dominant phyla varied with growth stages, dominant phyla Ascomycota and Chytridiomycota in AM5, dominant phyla Basidiomycota, Ascomycota and Zygomycota in BM5, and dominant phyla Basidiomycota and Ascomycota in CM5. There was no significant variation in the alpha-diversity of the bacterial and fungal communities, but significant variation was in the beta-diversity. We found that the variation of microbial community composition was accompanied by the changes in community function. The relative abundance of fungal pathogens increased with plant growth. We also identified the core microbes, significant-changing microbes, stage-specific microbes, and host-specific microbes. Plant weight, root length, root diameter, soil pH, rainfall, and climate temperature were the key divers to microbial community composition.
Our findings reported the variation and environmental drivers of rhizosphere bacterial and fungal communities during the growth of seedlings, which enhance the understanding of the rhizosphere microbial community in this habitat.
幼苗生长于高寒未开垦草甸土壤的雨养农业生态系统中，以确保幼苗移栽后的质量。目的是研究幼苗生长阶段根际细菌和真菌群落。
分别通过对16S和18S rDNA进行HiSeq测序来研究细菌和真菌群落。
变形菌门和拟杆菌门是整个生长阶段的细菌优势门类。真菌优势门类随生长阶段而变化，在AM5阶段优势门类为子囊菌门和壶菌门，在BM5阶段优势门类为担子菌门、子囊菌门和接合菌门，在CM5阶段优势门类为担子菌门和子囊菌门。细菌和真菌群落的α多样性无显著变化，但β多样性有显著变化。我们发现微生物群落组成的变化伴随着群落功能的改变。真菌病原体的相对丰度随植物生长而增加。我们还鉴定了核心微生物、显著变化微生物、阶段特异性微生物和宿主特异性微生物。植物重量、根长、根直径、土壤pH值、降雨量和气候温度是微生物群落组成的关键驱动因素。
我们的研究结果报道了幼苗生长期间根际细菌和真菌群落的变化及环境驱动因素，这增强了对该生境根际微生物群落的理解。
