Department of Plastic Surgery, King Edward Medical University, Lahore, Pakistan.
Rahber Medical and Dental College, Lahore, Pakistan.
Cell Biol Int. 2023 Jun;47(6):1118-1125. doi: 10.1002/cbin.12005. Epub 2023 Mar 1.
Facial contour deformities associated with pigmentary changes are of major concern for plastic surgeons being difficult to treat. No definite ideal treatment option is available to address simultaneously both the contour defects as well as related hyperpigmentation. The aim of the current study is to compare long-term effects of conventional adipose tissue grafting and ex-vivo expanded ASC-enriched adipose tissue grafting for the treatment of contour deformities related pigmentary changes on the face. In this study, 80 patients of contour deformities of face with hyperpigmentation were recruited after informed consent. Two techniques, that is, conventional fat grafting (C-FG) and fat grafts enriched with expanded adipose stem cells (FG-ASCs) were used to address the pigmentation. Both techniques were explained to patients and enrolled patients were divided into two groups, that is, C-FG and FG-ASCs as per patients' choice and satisfaction. Patients of the FG-ASCs group were treated with FG-ASCs while patients of C-FGs group were treated with C-FG (without expanded ASCs). Patients were followed for 12 months and improvement in face pigmentation was assessed clinically as well as measured objectively. Patients' satisfaction was also documented as highly satisfied, satisfied, and unsatisfied. This clinical trial was registered at www.clinicaltrials.gov with ID: NTC03564808. Mean age of patients was 24.42 (±4.49), 66 patients were females. Forehead was involved in 61.20% cases, cheek in 21.20% cases, chin in 11.20% cases, and nose in 6.20% cases. In GF-ASCs group, the integrated color density was decreased (1.08 × 10 ± 4.64 × 10 ) as compared with C-FG group (2.80 × 10 ± 1.69 × 10 ). Patients treated with fat grafts enriched with expanded ASCs were significantly more satisfied as compared with patients treated with C-FG only. In conclusion, ASC-enriched autologous fat grafting is preferred option for improving the contour deformities related increased pigmentation of face skin.
面部轮廓畸形伴色素改变是整形医生关注的主要问题，因为这些畸形较难治疗。目前尚无明确的理想治疗方案可以同时解决轮廓缺陷和相关的色素沉着问题。本研究旨在比较传统脂肪组织移植和体外扩增的 ASC 富脂肪组织移植治疗面部轮廓畸形相关色素改变的长期效果。
在这项研究中，经过知情同意，我们招募了 80 名面部轮廓畸形伴色素沉着的患者。我们采用了两种技术，即传统脂肪移植（C-FG）和富含扩增脂肪干细胞的脂肪移植（FG-ASCs）来解决色素沉着问题。向患者解释了这两种技术，并根据患者的选择和满意度将患者分为 C-FG 和 FG-ASCs 两组。
FG-ASCs 组的患者接受 FG-ASCs 治疗，C-FGs 组的患者接受 C-FG（不含扩增的 ASCs）治疗。患者随访 12 个月，临床和客观评估面部色素沉着的改善情况。同时记录患者的满意度，分为非常满意、满意和不满意。
这项临床试验已在 www.clinicaltrials.gov 注册，编号为 NTC03564808。患者的平均年龄为 24.42（±4.49）岁，其中 66 名为女性。额部受累 61.20%，颊部受累 21.20%，颏部受累 11.20%，鼻部受累 6.20%。
在 GF-ASCs 组，与 C-FG 组（2.80×10±1.69×10）相比，积分色密度降低（1.08×10±4.64×10）。与仅接受 C-FG 治疗的患者相比，接受富含 ASC 的自体脂肪移植治疗的患者满意度显著提高。
总之，富含 ASC 的自体脂肪移植是改善面部皮肤轮廓畸形相关色素沉着的首选方法。
