评价载蜂胶纳米粒正畸复合材料在大鼠动物模型中的抗菌效果。

Evaluation of antibacterial effect of the orthodontic composite containing propolis nanoparticles in rat as an animal model.

机构信息

Tehran University of Medical Sciences, Tehran, Iran.

出版信息

Folia Med (Plovdiv). 2023 Feb 28;65(1):131-139. doi: 10.3897/folmed.65.e67782.

DOI:10.3897/folmed.65.e67782

PMID:36855985

Abstract

AIM

The present study aimed to assess the antimicrobial effects of orthodontic primer containing nano-propolis against the cariogenic bacteria in а rat model.

MATERIALS AND METHODS

Transbond XT orthodontic primer containing 0%, 1%, 5%, and 10% nano-propolis was experimentally prepared in-house. The Wistar rats we used in the study were randomly divided into four groups and their oral cavities were colonized with Streptococcus mutans, Streptococcus sanguinis, and Lactobacillus acidophilus. After anesthetizing the rats, one drop (10 µL) of primer containing different concentrations of nano-propolis was applied to the labial surface of the maxillary incisor and light-cured. The orthodontic composite was applied on the primer and light-cured. One drop (10 µL) of primer containing the same concentrations of nano-propolis was again applied on the surface of composite and light-cured. The number of S. mutans, S. sanguinis, and L. acidophilus colonies in the saliva of rats was quantified at 24 h, at days 4 and 7 using plate counting.

RESULTS

Primer containing 1%, 5%, and 10% of nano-propolis significantly reduced the S. mutans colony count at 24 h compared with the control group (p<0.05). At day 4, the mean S. mutans colony counts in the 5% and 10% nano-propolis groups were significantly lower than that in the control group (p<0.05). Primer containing 1%, 5%, and 10% (all (p<0.05) of nano-propolis significantly reduced the L. acidophilus at 24 hours. Also, at day 4 the mean L. acidophilus colony counts in the 5% and 10% nano-propolis groups were significantly lower than that in the control group (p<0.05). At 24 h and 4 days, the mean S. sanguinis colony count in the 1%, 5%, and 10% nano-propolis group was significantly lower than that in the control group (p<0.05). No significant difference was observed in the presence of all concentrations of nano-propolis at day 7 (p>0.05).

CONCLUSIONS

Orthodontic primer containing nano-propolis significantly reduced the colony count of cariogenic bacteria in a rat model.

摘要

目的

本研究旨在评估含有纳米蜂胶的正畸底漆对致龋菌的抗菌作用。

材料和方法

本研究自行制备了含有 0%、1%、5%和 10%纳米蜂胶的 Transbond XT 正畸底漆。我们将用于研究的 Wistar 大鼠随机分为四组，其口腔定植变形链球菌、血链球菌和嗜酸乳杆菌。在麻醉大鼠后，将一滴（10μL）含有不同浓度纳米蜂胶的底漆应用于上颌切牙的唇面并光固化。将正畸复合树脂涂在底漆上并光固化。再次将一滴（10μL）含有相同浓度纳米蜂胶的底漆涂在复合树脂表面并光固化。在 24 小时、第 4 天和第 7 天使用平板计数法检测大鼠唾液中 S. mutans、S. sanguinis 和 L. acidophilus 菌落数量。

结果

与对照组相比，含有 1%、5%和 10%纳米蜂胶的底漆在 24 小时时显著降低了 S. mutans 菌落数（p<0.05）。第 4 天，5%和 10%纳米蜂胶组的 S. mutans 平均菌落数明显低于对照组（p<0.05）。含有 1%、5%和 10%纳米蜂胶（均 p<0.05）的底漆在 24 小时时显著降低了嗜酸乳杆菌。此外，在第 4 天，5%和 10%纳米蜂胶组的嗜酸乳杆菌平均菌落数明显低于对照组（p<0.05）。在 24 小时和第 4 天，1%、5%和 10%纳米蜂胶组的 S. sanguinis 平均菌落数明显低于对照组（p<0.05）。在第 7 天，所有浓度的纳米蜂胶均无明显差异（p>0.05）。