Huang Liyong, Dou Guangjian, Lu Jiajun, Chen Zhiheng, Wang Jiayi
Department of Gastrointestinal Surgery, The First Hospital of Jiaxing, Jiaxing, Zhejiang, China.
Department of Hepatobiliary Surgery, The First Hospital of Jiaxing, Jiaxing, Zhejiang, China.
Histol Histopathol. 2023 Dec;38(12):1443-1451. doi: 10.14670/HH-18-598. Epub 2023 Feb 23.
Colorectal cancer (CRC) is the third most commonly diagnosed cancer worldwide. A lack of effective targeted therapies against CRC makes the treatment challenging. Here, we report a circular RNA (circRNA), has_circ_0071803, functioning as an oncogene in CRC. Circ_0071803 was upregulated in CRC tissues and cell lines, and its expression levels were inversely correlated with the prognosis and survival rate of patients with CRC. Circ_0071803 knockdown suppressed cell proliferation, migration, and invasion in CRC. Moreover, we found that circ_0071803 sponged miR-330-5p, thereby upregulating mitogen-activated protein kinase 1 (MAPK1) in CRC cells. The suppression of cell activities by circ_0071803 knockdown were rescued by miR-330-5p inhibition or MAPK1 overexpression. Collectively, our findings elucidate that circ_0071803 promotes CRC progression by regulating the miR-330-5p/MAPK1 pathway, providing potential therapeutic targets for designing effective targeted treatments.
结直肠癌（CRC）是全球第三大常见诊断癌症。缺乏针对CRC的有效靶向治疗使得治疗具有挑战性。在此，我们报告一种环状RNA（circRNA），即has_circ_0071803，在CRC中作为一种癌基因发挥作用。Circ_0071803在CRC组织和细胞系中上调，其表达水平与CRC患者的预后和生存率呈负相关。敲低Circ_0071803可抑制CRC中的细胞增殖、迁移和侵袭。此外，我们发现circ_0071803可吸附miR-330-5p，从而上调CRC细胞中的丝裂原活化蛋白激酶1（MAPK1）。通过抑制miR-330-5p或过表达MAPK1可挽救敲低circ_0071803对细胞活性的抑制作用。总体而言，我们的研究结果表明，circ_0071803通过调节miR-330-5p/MAPK1途径促进CRC进展，为设计有效的靶向治疗提供了潜在的治疗靶点。
