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环状 RNA circ-FARSA 通过调控 miR-330-5p/LASP1 轴抑制结直肠癌细胞生长。

Knockdown of circular RNA circ-FARSA restricts colorectal cancer cell growth through regulation of miR-330-5p/LASP1 axis.

机构信息

Oncology Department, Henan Provincial People's Hospital, Zhengzhou, 450003, Henan province, China.

Oncology Department, Henan Provincial People's Hospital, Zhengzhou, 450003, Henan province, China.

出版信息

Arch Biochem Biophys. 2020 Aug 15;689:108434. doi: 10.1016/j.abb.2020.108434. Epub 2020 May 29.

DOI:10.1016/j.abb.2020.108434
PMID:32473899
Abstract

BACKGROUND

Circular RNA (circRNA) has been proposed to be involved in carcinogenesis. Here, we explored the functional significance and regulatory role of circ-FARSA in colorectal cancer (CRC).

METHODS

Gene expression was determined using quantitative reverse transcriptase polymerase chain reaction and Western blot. We determined the effect of circFARSA on CRC progression using cell count kit-8, colony formation assay, wound-healing assay, transwell invasion assay, luciferase reporter assay and in vivo assay.

RESULT

circ-FARSA was upregulated in CRC tissues and cell lines, and its expression had a significant association with the overall survival of CRC patients. Knockdown of circ-FARSA inhibited the proliferation, migration, and invasion of CRC cells in vitro. Moreover, circ-FARSA functioned as a sponge of miR-330-5p, and its upregulation mitigated the inhibitory effects of miR-330-5p on CRC cell proliferation and metastasis. In addition, circ-FARSA regulated the expression of LIM and SH3 protein 1 (LASP1) by sponging miR-330-5p. Besides, inhibition of circ-FARSA repressed the growth of CRC in vivo.

CONCLUSION

Silencing of circ-FARSA restricted the growth of CRC through regulating the miR-330-5p/LASP1 axis, providing a novel regulatory mechanism for CRC tumorigenesis.

摘要

背景

环状 RNA（circRNA）被认为参与了癌症的发生。在这里，我们探讨了 circ-FARSA 在结直肠癌（CRC）中的功能意义和调节作用。

方法

使用定量逆转录聚合酶链反应和 Western blot 测定基因表达。我们使用细胞计数试剂盒-8、集落形成测定、划痕愈合测定、Transwell 侵袭测定、荧光素酶报告测定和体内测定来确定 circFARSA 对 CRC 进展的影响。

结果

circ-FARSA 在 CRC 组织和细胞系中上调，其表达与 CRC 患者的总生存率有显著关联。circ-FARSA 的敲低抑制了 CRC 细胞在体外的增殖、迁移和侵袭。此外，circ-FARSA 作为 miR-330-5p 的海绵体发挥作用，其上调减轻了 miR-330-5p 对 CRC 细胞增殖和转移的抑制作用。此外，circ-FARSA 通过海绵 miR-330-5p 调节 LIM 和 SH3 蛋白 1（LASP1）的表达。此外，抑制 circ-FARSA 抑制了 CRC 在体内的生长。

结论

沉默 circ-FARSA 通过调节 miR-330-5p/LASP1 轴限制了 CRC 的生长，为 CRC 肿瘤发生提供了新的调节机制。

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