通过 MACS 从鼠乳腺肿瘤模型中同时分离 CD45 肿瘤浸润淋巴细胞、肿瘤细胞和相关成纤维细胞。
Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS.
机构信息
University Bourgogne Franche-Comté, 21000 Dijon, France; Cancer Biology Transfer Platform, Centre Georges-François Leclerc, 1 rue du professeur Marion, 21000 Dijon, France; Centre de Recherche INSERM LNC-UMR1231, 21000 Dijon, France; Genetic and Immunology Medical Institute, Dijon, France.
University Bourgogne Franche-Comté, 21000 Dijon, France; Department of Medical Oncology, Centre Georges-François Leclerc, 21000 Dijon, France; Cancer Biology Transfer Platform, Centre Georges-François Leclerc, 1 rue du professeur Marion, 21000 Dijon, France; Centre de Recherche INSERM LNC-UMR1231, 21000 Dijon, France; Genetic and Immunology Medical Institute, Dijon, France.
出版信息
STAR Protoc. 2023 Mar 17;4(1):101951. doi: 10.1016/j.xpro.2022.101951. Epub 2023 Jan 19.
The study of the tumor microenvironment (TME) and its interactions with cancer cells is an important issue in cancer research. Here, we present a protocol to sort three important cell populations from murine triple negative breast cancer 4T1 model TME, including CD45 tumor-infiltrating lymphocytes, cancer-associated fibroblasts, and tumor cells. The protocol includes four steps: generation of 4T1 tumors, tumor collection and digestion, magnetic sorting of the different populations, and phenotypic validation of sorted cells. For complete details on the use and execution of this protocol, please refer to Limagne et al. (2022)..
研究肿瘤微环境（TME）及其与癌细胞的相互作用是癌症研究中的一个重要问题。在这里，我们提出了一种从鼠源三阴性乳腺癌 4T1 模型 TME 中分离三种重要细胞群体的方案，包括 CD45 肿瘤浸润淋巴细胞、癌相关成纤维细胞和肿瘤细胞。该方案包括四个步骤：生成 4T1 肿瘤、肿瘤收集和消化、不同群体的磁性分选以及分选细胞的表型验证。有关该方案使用和执行的完整详细信息，请参考 Limagne 等人（2022 年）。。
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