School of Chemistry and Chemical Engineering, Guangxi Minzu University; Key Laboratory of Chemistry and Engineering of Forest Products, State Ethnic Affairs Commission; Guangxi Key Laboratory of Chemistry and Engineering of Forest Products; Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products; Key Laboratory of Applied Analytical Chemistry (Guangxi Minzu University), Education Department of Guangxi Zhuang Autonomous Region, Nanning 530006, China.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, P. R. China.
Analyst. 2023 Mar 13;148(6):1362-1370. doi: 10.1039/d3an00024a.
Exploring new highly efficient electrochemiluminescence (ECL) luminophores is a necessary condition for developing ultrasensitive ECL biosensors. Therefore, a luminescent carbon dot-based covalent organic framework (CD-COF) was prepared using aldehyde-based carbon dots (CDs) and 1,3,5-tris (4-aminophenyl) benzene (TPB). Because the CD-COF made the regular arrangement of CDs conducive to improving the ECL response, CD-COF had a higher ECL intensity and efficiency than CDs. What's more, the ECL intensity of the CD-COF/SO/BuN system was about 2.98, 7.50, and 28.08 times higher than those of the CD-COF/SO, CDs/SO and SO systems, respectively. Considering the remarkable ECL performance, the CD-COF/SO/BuN system was employed combined with the CRISPR/Cas12a trans-cutting strategy to construct an "off-on" ECL biosensor for BPA detection. The proposed ECL biosensor exhibited excellent performance with a wide linear range from 1.0 × 10 mol L to 1.0 × 10 mol L with a low detection limit of 2.21 fM (S/N = 3) under the optimized conditions. The biosensor demonstrated that CD-COF can be used as an efficient ECL emitter, thus expanding the application field of COFs. In addition, the good stability and specificity of the biosensor enabled the rapid detection of BPA, which will provide valuable insights into promising ultrasensitive ECL biosensors.
探索新型高效电致化学发光（ECL）发光体是开发超灵敏 ECL 生物传感器的必要条件。因此，使用基于醛的碳点（CDs）和 1,3,5-三（4-氨基苯基）苯（TPB）制备了基于发光碳点的共价有机框架（CD-COF）。由于 CD-COF 使 CDs 的规则排列有利于提高 ECL 响应，因此 CD-COF 具有比 CDs 更高的 ECL 强度和效率。更重要的是，CD-COF/SO/BuN 体系的 ECL 强度分别比 CD-COF/SO、CDs/SO 和 SO 体系高约 2.98、7.50 和 28.08 倍。考虑到显著的 ECL 性能，将 CD-COF/SO/BuN 体系与 CRISPR/Cas12a 转切策略结合，构建了用于 BPA 检测的“关-开”ECL 生物传感器。在优化条件下，所提出的 ECL 生物传感器表现出优异的性能，线性范围从 1.0×10 到 1.0×10 ，检测限低至 2.21 fM（S/N = 3）。该生物传感器表明 CD-COF 可用作高效的 ECL 发射器，从而扩展了 COFs 的应用领域。此外，生物传感器具有良好的稳定性和特异性，能够快速检测 BPA，这将为有前途的超灵敏 ECL 生物传感器提供有价值的见解。
