Department of Microbiology & Immunology, Cornell University, Ithaca, NY 14853, USA.
3M Company, St. Paul, MN 55144, USA.
Immunobiology. 2023 May;228(3):152356. doi: 10.1016/j.imbio.2023.152356. Epub 2023 Feb 22.
Perfluorooctane sulfonic acid (PFOS) is member of a class of molecules with fluorinated carbon chains known as polyfluoroalkyls. PFOS have been used to produce a variety of industry and comsumer uses. However, a significant concern is that it accumulates in the environment, including in animals and humans, and that it is a potential immunosuppressant. Here we analyze immune homeostasis in mice following chronic exposure to PFOS at levels up to those historically found in PFOS manufacturing workers. Mice were exposed to 0.15, 1.5, 15, or 50 µg /kg of PFOS for 28 days, after which, B cells, T cells, and granulocytes from the bone marrow, liver, spleen, lymph nodes, and thymus were evaluated. We find that at these exposures, there was no effect of PFOS on major T- or B-cell populations, macrophages, dendritic cells, basophils, mast cells, eosinophils, neutrophils, serum antibodies or select serum cytokines. By contrast, mice exposed the known immunosuppressant cyclophosphamide, which was given at 40 mg/kg for four days, exhibited depletion of several granulocyte, T- and B-cell populations of the thymus, bone marrow, and spleen, as well as circulating IgM and IgE antibodies. These data indicate that exposures of up to 50 µg /kg of PFOS for 28 days does not affect immune homeostasis in mice.
全氟辛烷磺酸 (PFOS) 是一类具有氟化碳链的分子的成员，被称为全氟烷基物质。PFOS 已被用于生产各种工业和消费品。然而，人们非常关注的是，它会在环境中积累，包括在动物和人类体内，而且它可能是一种潜在的免疫抑制剂。在这里，我们分析了在慢性暴露于 PFOS 水平高达 PFOS 制造工人历史水平的情况下，小鼠的免疫稳态。小鼠暴露于 0.15、1.5、15 或 50μg/kg 的 PFOS 28 天，之后评估了来自骨髓、肝脏、脾脏、淋巴结和胸腺的 B 细胞、T 细胞和粒细胞。我们发现，在这些暴露水平下，PFOS 对主要的 T 或 B 细胞群体、巨噬细胞、树突状细胞、嗜碱性粒细胞、肥大细胞、嗜酸性粒细胞、中性粒细胞、血清抗体或特定的血清细胞因子没有影响。相比之下，暴露于已知免疫抑制剂环磷酰胺的小鼠（连续四天给予 40mg/kg）表现出胸腺、骨髓和脾脏中几种粒细胞、T 细胞和 B 细胞群体以及循环 IgM 和 IgE 抗体的耗竭。这些数据表明，在 28 天内暴露于高达 50μg/kg 的 PFOS 不会影响小鼠的免疫稳态。
