Department of Microbiology & Immunology, Cornell University, Ithaca, NY 14853, USA.
3M Company, St. Paul, MN 55144, USA.
Immunol Lett. 2021 May;233:31-41. doi: 10.1016/j.imlet.2021.03.006. Epub 2021 Mar 12.
Perfluoroctanesulfonate (PFOS) belongs to a larger family of compounds known as Per- and polyfluoroalkyl substances (PFAS). The strength of the carbon-fluorine bond makes PFOS extremely resistant to environmental degradation. Due to its persistent nature, research has been directed to elucidating possible health effects of PFOS on humans and laboratory animals. Here we have explored the effects of PFOS exposure on immune development and function in mice. We exposed adult mice to 3 and 1.5 μg/kg/day of PFOS for 2 and 4 weeks, respectively, and examined the effects of PFOS exposure on populations of T cells, B cells, and granulocytes. These doses of PFOS resulted in serum levels of approximately 100 ng/mL with no weight loss during exposure. We find that PFOS does not affect T-cell development during this time. However, while PFOS exposure reduced immune cell populations in some organs, it also led to an increase in the numbers of cells in others, suggesting possible relocalization of cells. We also examined the effect of PFOS on the response to influenza virus infection. We find that exposure to PFOS at 1.5 μg/kg/day of PFOS for 4 weeks does not affect weight loss or survival, nor is viral clearance affected. Analysis of antibody and T cell specific antiviral responses indicate that at this concentration, PFOS does not suppress the immune cell development or antigen specific immune response.
全氟辛烷磺酸 (PFOS) 属于全氟和多氟烷基物质 (PFAS) 这一大类化合物。碳氟键的强度使得 PFOS 极难在环境中降解。由于其持久性，研究已经针对 PFOS 对人类和实验动物可能产生的健康影响展开。在这里，我们研究了 PFOS 暴露对小鼠免疫发育和功能的影响。我们分别以 3 和 1.5 μg/kg/天的剂量使成年小鼠暴露于 PFOS 2 和 4 周，并检查了 PFOS 暴露对 T 细胞、B 细胞和粒细胞群体的影响。这些剂量的 PFOS 导致血清水平约为 100 ng/mL，在暴露期间没有体重减轻。我们发现 PFOS 在此期间不会影响 T 细胞的发育。然而，虽然 PFOS 暴露减少了某些器官中的免疫细胞群体，但它也导致了其他器官中细胞数量的增加，表明细胞可能发生了重新定位。我们还研究了 PFOS 对流感病毒感染反应的影响。我们发现，以 1.5 μg/kg/天的剂量暴露于 PFOS 4 周不会影响体重减轻或存活率，也不会影响病毒清除。对抗体和 T 细胞抗病毒反应的分析表明，在该浓度下，PFOS 不会抑制免疫细胞的发育或抗原特异性免疫反应。
