Satria Denny, Harahap Urip, Dalimunthe Aminah, Septama Abdi Wira, Hertiani Triani, Nasri Nasri
Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Jl Tri Dharma No. 5 Kampus USU, Medan 20155, Sumatera Utara, Indonesia.
Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Jl Tri Dharma No. 5 Kampus USU, Medan 20155, Sumatera Utara, Indonesia.
Adv Pharmacol Pharm Sci. 2023 Feb 20;2023:2259534. doi: 10.1155/2023/2259534. eCollection 2023.
Multidrug-resistant bacteria have raised global concern about the inability to fight deadly infectious diseases. Methicillin-resistant (MRSA) and are the most common resistant bacteria that are causing hospital infections. The present study was undertaken to investigate the synergistic antibacterial effect of the ethyl acetate fraction of Delile leaves (EAFVA) with tetracycline against the clinical isolates MRSA and . Microdilution was used to establish the minimum inhibitory concentration (MIC). A checkerboard assay was conducted for the interaction effect. Bacteriolysis, staphyloxanthin, and a swarming motility assay were also investigated. EAFVA exhibited antibacterial activity against MRSA and with a MIC value of 125 g/mL. Tetracycline showed antibacterial activity against MRSA and with MIC values of 15.62 and 31.25 g/mL, respectively. The interaction between EAFVA and tetracycline showed a synergistic effect against MRSA and with a Fractional Inhibitory Concentration Index (FICI) of 0.375 and 0.31, respectively. The combination of EAFVA and tetracycline induced the alteration of MRSA and , leading to cell death. Moreover, EAFVA also inhibited the quorum sensing system in MRSA and The results revealed that EAFVA enhanced the antibacterial activity of tetracycline against MRSA and . This extract also regulated the quorum sensing system in the tested bacteria.
多重耐药细菌引发了全球对无法对抗致命传染病的担忧。耐甲氧西林金黄色葡萄球菌（MRSA）是导致医院感染的最常见耐药细菌。本研究旨在探讨德利叶乙酸乙酯提取物（EAFVA）与四环素对临床分离的MRSA的协同抗菌作用。采用微量稀释法确定最低抑菌浓度（MIC）。进行棋盘法检测相互作用效果。还研究了细菌溶解、金黄色葡萄球菌素和群集运动试验。EAFVA对MRSA表现出抗菌活性，MIC值为125μg/mL。四环素对MRSA的MIC值分别为15.62和31.25μg/mL，表现出抗菌活性。EAFVA与四环素之间的相互作用对MRSA表现出协同作用，分数抑菌浓度指数（FICI）分别为0.375和0.31。EAFVA与四环素的组合诱导了MRSA的改变，导致细胞死亡。此外，EAFVA还抑制了MRSA中的群体感应系统。结果表明，EAFVA增强了四环素对MRSA的抗菌活性。该提取物还调节了受试细菌中的群体感应系统。
