Kuo I-Pei, Liu Ching-Shuo, Yang Shuenn-Der, Liang Shih-Hsiang, Hu Yeh-Fang, Nan Fan-Hua
Freshwater Aquaculture Research Center Chupei Station, Fisheries Research Institute, Council of Agriculture, No. 111, Tai-Ho, Zhubei, Hsinchu 30267, Taiwan.
Department of Aquaculture, National Taiwan Ocean University, No. 2, Beining Road, Keelung 202301, Taiwan.
Aquac Nutr. 2022 Dec 1;2022:1866142. doi: 10.1155/2022/1866142. eCollection 2022.
An 8-week feeding trial was conducted to investigate the effects of replacing fishmeal with defatted black soldier fly larvae meal (DBSFLM) in the diets of Japanese eel on their growth performance, fillet texture, serum biochemical parameters, and intestinal histomorphology. Six isoproteic (520 g kg), isolipidic (80 g kg), and isoenergetic (15 MJ kg) diets were formulated with fishmeal replacement levels of 0% (R0), 15% (R15), 30% (R30), 45% (R45), 60% (R60), and 75% (R75). The growth performance, feed utilization efficiency, survival rate, serum liver function enzymes, antioxidant ability, and lysozyme activity of fish were not affected ( > 0.05) by DBSFLM. However, the crude protein and cohesiveness of the fillet in groups R60 and R75 significantly decreased, and the fillet hardness significantly increased ( < 0.05). Additionally, the intestinal villus length significantly decreased in the R75 group, and the goblet cell densities were significantly lower in the R45, R60, and R75 groups ( < 0.05). Overall, high levels of DBSFLM did not affect growth performance and serum biochemical parameters but significantly altered fillet proximate composition and texture and intestinal histomorphology ( < 0.05). The optimal fishmeal replacement level is 30% with 184 g kg DBSFLM.
进行了一项为期8周的饲养试验，以研究用脱脂黑水虻幼虫粉（DBSFLM）替代日本鳗鱼日粮中的鱼粉对其生长性能、鱼片质地、血清生化参数和肠道组织形态的影响。配制了六种等蛋白（520 g/kg）、等脂（80 g/kg）和等能（15 MJ/kg）的日粮，鱼粉替代水平分别为0%（R0）、15%（R15）、30%（R30）、45%（R45）、60%（R60）和75%（R75）。DBSFLM对鱼的生长性能、饲料利用效率、存活率、血清肝功能酶、抗氧化能力和溶菌酶活性没有影响（P>0.05）。然而，R60组和R75组鱼片的粗蛋白含量和内聚性显著降低，鱼片硬度显著增加（P<0.05）。此外，R75组的肠绒毛长度显著降低，R45、R60和R75组的杯状细胞密度显著降低（P<0.05）。总体而言，高水平的DBSFLM对生长性能和血清生化参数没有影响，但显著改变了鱼片的近似成分、质地和肠道组织形态（P<0.05）。最佳鱼粉替代水平为30%，即184 g/kg DBSFLM。
