Xia Dongfang, Zhang Yong, Zhang Chunyu, Yao Xiuxiu, Tang Yuhua, Wang Fuchao, Han Xu, Yin Hongzong, Xu Chao, Gao Xueyun
College of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong, China.
Faculty of Environment and Life, Beijing University of Technology, Beijing, China.
Front Chem. 2023 Feb 13;11:1145415. doi: 10.3389/fchem.2023.1145415. eCollection 2023.
is overexpressed in various tumor cells and plays a key role in tumor genesis, invasion, and metastasis. Therefore, it is of great significance to precisely detect the level in cells via a simple method. For this purpose, we have constructed a peptide-coated platinum (Pt) cluster. Due to its bright fluorescence, well-defined Pt atom numbers, and peroxidase-like catalytic activity, this cluster can be used to evaluate levels in cells by fluorescence imaging, inductively coupled plasma mass spectrometry (ICP-MS), and catalytic amplification of visual dyes, respectively. In this report, the expression level of in living cells is well-detected by the naked eye under an ordinary light microscope when the Pt cluster binds to αvβ3 in cells and catalyzes non-color 3,3'-diaminobenzidine (DAB) into brown-colored molecules . Moreover, SiHa, HeLa, and 16HBE cell lines with different expression levels can be visually distinguished by the peroxidase-like Pt clusters. This research will provide a reliable method for the simple detection of levels in cells.
在各种肿瘤细胞中过表达，并在肿瘤发生、侵袭和转移中起关键作用。因此，通过一种简单的方法精确检测细胞中的水平具有重要意义。为此，我们构建了一种肽包被的铂（Pt）簇。由于其明亮的荧光、明确的铂原子数和类过氧化物酶催化活性，该簇可分别用于通过荧光成像、电感耦合等离子体质谱（ICP-MS）和可视染料的催化扩增来评估细胞中的水平。在本报告中，当Pt簇与细胞中的αvβ3结合并将无色的3,3'-二氨基联苯胺（DAB）催化成棕色分子时，在普通光学显微镜下用肉眼可以很好地检测活细胞中的表达水平。此外，具有不同表达水平的SiHa、HeLa和16HBE细胞系可以通过类过氧化物酶的Pt簇进行视觉区分。本研究将为简单检测细胞中的水平提供一种可靠的方法。
